Ai65(RCFL-tdT) targeting vector
PurposeTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61577||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneRosa26 Targeting Vector
- Backbone size w/o insert (bp) 2881
- Total vector size (bp) 16295
Vector typeMouse Targeting, Cre/Lox
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsGrow in 15 microg/ml kanamycin
Copy numberHigh Copy
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site Sac1 (not destroyed)
- 3′ cloning site Cla (not destroyed)
- 5′ sequencing primer TCTTCGCTATTACGCCAGCT
- 3′ sequencing primer AACAGCTATGACCATG (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
NOTE: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. The depositing lab has assessed these differences and states that they do no affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ai65(RCFL-tdT) targeting vector was a gift from Hongkui Zeng (Addgene plasmid # 61577 ; http://n2t.net/addgene:61577 ; RRID:Addgene_61577)
For your References section:Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H. Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. 10.1016/j.neuron.2015.02.022 PubMed 25741722