252,515 results

1. plenti-UbcP-FLAG-CRBN-pGK-HYG

   Plasmid

   #107374

   Purpose

   Lentiviral expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-UbcP-FLAG-CRBN-pGK-HYG

   Plasmid

   #107374

   Purpose

   Lentiviral expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-MCS1-P2A-MCS2 (Blast)

   Plasmid

   #80921

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW57-MCS1-P2A-MCS2 (Blast)

   Plasmid

   #80921

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

   Plasmid

   #171019

   Purpose

   Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   LargeBit-SNAPtag- Beta-actin
   

   Use

   Lentiviral and Luciferase

   Tags

   Beta actin and LargeBiT

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEB3-mBaoJin

   Plasmid

   #229565

   Purpose

   Expresses EB3-mBaoJin fusion in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

   Insert

   mBaoJin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCJ190

    Plasmid

    #162667

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCh-Rab5

    Plasmid

    #49201

    Purpose

    expression of Rab5 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    Rab5 (RAB5B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCh-Rab5

    Plasmid

    #49201

    Purpose

    expression of Rab5 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    Rab5 (RAB5B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Use

    Tags

    mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCV-Cas9

    Plasmid

    #123643

    Purpose

    Expresses Cas9 with an N-terminal HUH-tag called PCV2

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Use

    Tags

    (EAAAK)8 linker and His6 + SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge