166,818 results

1. pAAV-hSyn-hChR2(H134R)-mCherry

   Plasmid

   #26976

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV5, AAV8, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-14-3-3 gamma

   Plasmid

   #13274

   Depositor

   Michael Yaffe

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-R-diLACCO1

   Plasmid

   #208028

   Purpose

   Control biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-diLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PIK3C2A

   Plasmid

   #161988

   Purpose

   Mammalian expression vector containing PIK3C2A tagged with GFP

   Depositor

   Volker Haucke, David Bryant

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5HRE/GFP

   Plasmid

   #46926

   Purpose

   hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

   Depositor

   Martin Brown, Thomas Foster

   Article

   Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Use

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   five copies of a 35-bp fragment from the hypoxia-…

   Promoter

   EF-1α promoter

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. A2_uSFFV

   Plasmid

   #153415

   Purpose

   Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   A*02:01-P2A-human beta2 microglobulin (B2M Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 flag p53

   Plasmid

   #10838

   Depositor

   Thomas Roberts

   Article

   Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_FASN

   Plasmid

   #107138

   Purpose

   His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Insert

   Fatty acid synthase (FASN Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAX APLP1

    Plasmid

    #30141

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Like Protein 1 (APLP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pT7-TaGu-WT-3xF-ORF

    Plasmid

    #238487

    Purpose

    For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available Since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223_CBFB_WT

    Plasmid

    #81929

    Purpose

    Gateway Donor vector containing CBFB, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CBFB (CBFB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2531

    Plasmid

    #142954

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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