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pcDNA3 HA-PHLPP1 full length
(Plasmid #37100)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37100 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PHLPP1
  • Alt name
    SCOP
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_194449
  • Entrez Gene
    PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the insert in this plasmid was referred to as PHLPP1beta in the original publication (Brognard et al., Mol. Cell 2007) and the plasmid was first described in Warfel et al. 2011.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA-PHLPP1 full length was a gift from Alexandra Newton (Addgene plasmid # 37100 ; http://n2t.net/addgene:37100 ; RRID:Addgene_37100)
  • For your References section:

    Mislocalization of the E3 ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), in glioblastoma uncouples negative feedback between the pleckstrin homology domain leucine-rich repeat protein phosphatase 1 (PHLPP1) and Akt. Warfel NA, Niederst M, Stevens MW, Brennan PM, Frame MC, Newton AC. J Biol Chem. 2011 Jun 3;286(22):19777-88. Epub 2011 Mar 28. 10.1074/jbc.M111.237081 PubMed 21454620