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pEGFP(C1)-PP1gamma
(Plasmid #44225)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44225 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5672
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP-1G
  • Alt name
    PPP1CC
  • Alt name
    PPP1CC protein phosphatase 1, catalytic subunit, gamma isozyme
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    972
  • GenBank ID
    NM_002710
  • Entrez Gene
    PPP1CC (a.k.a. PP-1G, PP1C, PPP1G)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer TGGTCCTGCTGGAGTTCGTGACC
  • 3′ sequencing primer GTTTGGACAAACCACAACTAGAATGCAG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that there is a silent mutation at bp#784 when compared to GenBank reference sequence NM_002710.3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP(C1)-PP1gamma was a gift from Angus Lamond & Laura Trinkle-Mulcahy (Addgene plasmid # 44225 ; http://n2t.net/addgene:44225 ; RRID:Addgene_44225)
  • For your References section:

    Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells. Trinkle-Mulcahy L, Sleeman JE, Lamond AI. J Cell Sci. 2001 Dec;114(Pt 23):4219-28. PubMed 11739654