pAc-C Med12His
(Plasmid #49240)

• Purpose: expresses human Med12 with His tag in insect cells
• Depositing Lab: Dylan Taatjes
• Publication: Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. doi: 10.1128/MCB.00993-08. Epub 2008 Dec 1.

Backbone

• Vector backbone: pAC-GHLT
• Backbone manufacturer: Pharmingen
• Backbone size w/o insert (bp): 8753
• Modifications to backbone: modified to contain no GST tag and a C-terminal His tag.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Med12
• Alt name: mediator complex subunit 12
• Species: H. sapiens (human)
• Entrez Gene: MED12 (a.k.a. ARC240, CAGH45, FGS1, HDKR, HOPA, Kto, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230)
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: polyhedrin forward
• 3′ sequencing primer: Baculovirus_rev_primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry