168,814 results

1. pET-32a(+)-TGFB1m

   Plasmid

   #120286

   Purpose

   Produce human recombinant mTGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β1
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promotor

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pwB2b

   Plasmid

   #32094

   Depositor

   Christopher Buck

   Article

   Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

   Inserts

   BKV VP1
   BKV VP2
   

   Expression

   Mammalian

   Mutation

   codon modified

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGK-empty

   Plasmid

   #35094

   Depositor

   David Segal

   Article

   Bhakta et al Methods Mol Biol. 2010;649:3-30.

   Insert

   deletion of zinc fingers and nuclease
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   use as a transfection balance for pPGK-ZFN plasmi…

   Promoter

   PGK

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 TFAM-mClover

   Plasmid

   #129574

   Purpose

   Expression of mClover fused TFAM

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   TFAM (TFAM Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (AAV8)

   Viral Prep

   #137154-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (#137154). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of NpHR3.3-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

6. CS-TRE-c-Myc-Ubc-rtTA

   Plasmid

   #198190

   Purpose

   Generation of Expandable megakaryocyte cell lines from human iPS cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

   Insert

   hC-MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDB179-HotPETase

   Plasmid

   #218701

   Purpose

   Encodes codon optimized HotPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   HotPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

   Plasmid

   #175525

   Purpose

   Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAX_CAGGS_CAS9_mcherry

   Plasmid

   #167862

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-UBC-XRI-HA

    Plasmid

    #178056

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-HA
    

    Use

    AAV

    Tags

    HA-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM60 FLAG-RAB35 wt

    Plasmid

    #107104

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Promoter

    Simian CMV IE94

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

    Plasmid

    #238234

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoA
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV Retrograde)

    Viral Prep

    #162377-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pEGFP-STAG2-wildtype

    Plasmid

    #31972

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-NurRE3

    Plasmid

    #208253

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X Nur response element LUC
    

    Tags

    No

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORTMAGE-3

    Plasmid

    #72678

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

    Plasmid

    #40343

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    RSV promoter
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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