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High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening.
Gier RA, Budinich KA, Evitt NH, Cao Z, Freilich ES, Chen Q, Qi J, Lan Y, Kohli RM, Shi J
Nat Commun. 2020 Jul 13;11(1):3455. doi: 10.1038/s41467-020-17209-1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
149722EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)Lentiviral expression of crRNA with GFP and a neomycin resistance gene
149723AsCas12a-6xNLS-E174R/S542R (pRG232)Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

Antibodies from Article