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Flag-Daxx/pRK5

Plasmid

#27974

Depositor

Xiaolu Yang

Article

Tang et al Nat Cell Biol. 2006 Aug . 8(8):855-62.

Insert

Daxx (DAXX Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
HA-TRIM28deltaPB

Plasmid

#124959

Purpose

Mammalian expression of Human TRIM28 with deleted PHD and BROMO domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of PHD and Bromo Domain

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dPCP

Plasmid

#231090

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

mNG-IRES-mScarlet-dPCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ACTN2-pEGFP

Plasmid

#52669

Purpose

Overexpression of GFP-tagged α-actinin-2

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

actinin 2 (ACTN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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RNA-binding protein pooled CRISPR knockout library

Pooled Library

#141438

Purpose

Knockout library targeting RNA-binding proteins with 10 guide RNAs per target.

Depositor

Eugene Yeo

Article

Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only
plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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ADORA2B-Tango

Plasmid

#66211

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADORA2B (ADORA2B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDB5320

Plasmid

#204830

Purpose

An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW2 promoter

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-LK03

Plasmid

#206512

Purpose

AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

Insert

LK03 capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSSRB274_pAC8-eGFP-hsCRBN

Plasmid

#218791

Purpose

Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Tags

FLAG-TEV-eGFP-Prescission

Expression

Insect

Mutation

eGFP fused to N-terminus

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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LAMP1-APEX2

Plasmid

#215555

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMP1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Cox8A Control Plasmid

Plasmid

#101129

Purpose

Expression of SNAP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRetroX-TetOne-Puro-p62

Plasmid

#204548

Purpose

Expresses p62 in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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TRE3G-PE2-P2A-BFP

Plasmid

#231580

Purpose

Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

Depositor

Jesse Engreitz

Article

Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

Insert

PE2-P2A-BFP

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV CreERT2 puro

Plasmid

#22776

Purpose

Retroviral expression of tamoxifen-inducible Cre-ERT2

Depositor

Tyler Jacks

Article

Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pSLS.400

Plasmid

#187201

Purpose

Expresses retron Eco4 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco4 Reverse Transcriptase

Use

Synthetic Biology

Expression

Bacterial

Promoter

mphR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits