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p2xFlag CMV2-YAP2-1st&2nd WW mutant
(Plasmid #19048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19048 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 19048-D50 50 μg of DNA in Tris buffer $495
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DNA 19048-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p2xFlag CMV2
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Yes-kinase associated protein
  • Alt name
    YAP2, YAP65
  • Alt name
    YAP2 isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1467
  • Mutation
    Tryptophan 199 changed to Alanine,and Proline 202 changed to Alanine, andTryptophan 258 changed to Alanine,and Proline 261 changed to Alanine
  • GenBank ID
    NP_001181973.1
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Tag / Fusion Protein
    • 2xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2xFlag CMV2-YAP2-1st&2nd WW mutant was a gift from Marius Sudol (Addgene plasmid # 19048 ; http://n2t.net/addgene:19048 ; RRID:Addgene_19048)

  • For your References section:

    Mst2 and Lats kinases regulate apoptotic function of Yes kinase-associated protein (YAP). Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 10.1074/jbc.M804380200. Epub 2008 Jul 17. 10.1074/jbc.M804380200 PubMed 18640976
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