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Addgene

p3xFLAG-CMV14-mULK1
(Plasmid #24301)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV14
  • Backbone manufacturer
    sigma-aldrich
  • Backbone size w/o insert (bp) 6299
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a novel protein kinase structurally related to C. elegans UNC-51
  • Alt name
    ULK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3614
  • GenBank ID
    AF053756
  • Entrez Gene
    Ulk1 (a.k.a. AU041434, Unc51.1, mKIAA0722)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV 30 sequencing primer
  • 3′ sequencing primer CMV 24 sequencing primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV14-mULK1 was a gift from Noboru Mizushima (Addgene plasmid # 24301 ; http://n2t.net/addgene:24301 ; RRID:Addgene_24301)

  • For your References section:

    Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N. Mol Biol Cell. 2009 Apr . 20(7):1981-91. 10.1091/mbc.e08-12-1248 PubMed 19211835
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