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Addgene

p3xFLAG-CMV10-hAtg101
(Plasmid #22877)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6299
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Atg13-interacting protein
  • Alt name
    ATG101
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    657
  • Entrez Gene
    ATG101 (a.k.a. C12orf44)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV 30 sequencing primer
  • 3′ sequencing primer CMV 24 sequencing primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV10-hAtg101 was a gift from Noboru Mizushima (Addgene plasmid # 22877 ; http://n2t.net/addgene:22877 ; RRID:Addgene_22877)

  • For your References section:

    Atg101, a novel mammalian autophagy protein interacting with Atg13. Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N. Autophagy. 2009 Oct . 5(7):973-9. 10.4161/auto.5.7.9296 PubMed 19597335
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