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HA-EglN2-pcDNA3
(Plasmid #18961)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18961 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5445
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Egg laying Nine - 2
  • Alt name
    PHD1
  • Alt name
    prolyl Hydroxylase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1227
  • Mutation
    wt cDNA
  • GenBank ID
    BC036051
  • Entrez Gene
    EGLN2 (a.k.a. EIT-6, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-EglN2-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18961 ; http://n2t.net/addgene:18961 ; RRID:Addgene_18961)
  • For your References section:

    Neuronal apoptosis linked to EglN3 prolyl hydroxylase and familial pheochromocytoma genes: developmental culling and cancer. Lee S, Nakamura E, Yang H, Wei W, Linggi MS, Sajan MP, Farese RV, Freeman RS, Carter BD, Kaelin WG, Schlisio S. Cancer Cell. 2005 Aug . 8(2):155-67. 10.1016/j.ccr.2005.06.015 PubMed 16098468