|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17972||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typemycobacteria expression
Growth in Bacteria
Bacterial Resistance(s)Hygromycin, 200 μg/mL
Growth instructionshygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria). This plasmid is high copy number in E. coli and low copy number in mycobacteria.
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379.
Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSE100 was a gift from Sabine Ehrt (Addgene plasmid # 17972 ; http://n2t.net/addgene:17972 ; RRID:Addgene_17972)
For your References section:Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222