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pUV15tetORm
(Plasmid #17975)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17975 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMS2
  • Vector type
    mycobacteria expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria. This plasmid is high copy number in E. coli and low copy number in mycobacteria.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pmyc1tetO-gfp, tetR(B)
  • Alt name
    GFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.

pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUV15tetORm was a gift from Sabine Ehrt (Addgene plasmid # 17975 ; http://n2t.net/addgene:17975 ; RRID:Addgene_17975)
  • For your References section:

    Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222