260,515 results

1. pcDNA5/FRT_mEGFP-SNAP-tag2

   Plasmid

   #226513

   Purpose

   Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   mEGFP-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. huCaV2.2 (pSAD442-1)

   Plasmid

   #62574

   Purpose

   Human CaV2.2 expression in mammalian cells

   Depositor

   Diane Lipscombe

   Article

   Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

   Insert

   CACNA1B (CACNA1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-Cre-P2A-dTomato (AAV8)

   Viral Prep

   #107738-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Rylan Larsen

   Promoter

   Syn

   Tags

   dTomato (physically separate, not a fusion protein)

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. c-Fos

   Plasmid

   #187905

   Purpose

   Expresses c-Fos in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   cFos (FOS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbA5a-sufCDSUB

   Plasmid

   #195055

   Purpose

   Expresses SUF operon from B. subtilis from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufCDSUB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQC-CherryOFF-GFP

   Plasmid

   #129101

   Purpose

   Reporter vector

   Depositor

   Fangliang Zhang

   Article

   Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

   Insert

   CherryOFF
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G-> A

   Promoter

   CMV

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-ShadowG

   Plasmid

   #104620

   Purpose

   Expresses Shadow G in mammalian cell

   Depositor

   Hideji Murakoshi

   Article

   Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

   Insert

   ShadowG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAG-BirA*-dCas9-eGFP

    Plasmid

    #84779

    Purpose

    Expresses BirA*-dCas9-eGFP in mammalian cells.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    BirA*-dCas9-eGFP
    

    Use

    CRISPR

    Tags

    cMyc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV5)

    Viral Prep

    #105547-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Dio-GFP

    Plasmid

    #198279

    Purpose

    Cre reporter for mammalian cells; double-floxxed-EGFP

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Dio-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pHAGE NFkB-TA-LUC-UBC-GFP-W

    Plasmid

    #49343

    Purpose

    pHAGE NFkB-TA-LUC-UBC-GFP-W

    Depositor

    Darrell Kotton

    Article

    Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

    Inserts

    eGFP (eGFP )
    Luciferase
    NFkB consensus binding sequence
    TA
    UBC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-jGCaMP8s-WPRE (AAV1)

    Viral Prep

    #179256-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-CAG-jGCaMP8s-WPRE (#179256). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8s-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Tags

    None

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pLKO5.sgRNA.EFS.PAC

    Plasmid

    #57825

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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