181,097 results
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Plasmid#100943PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1aDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHAExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility
Pooled library#101931PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCVpuro-eGFP-mcGAS
Plasmid#108675PurposeFor production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGASDepositorAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CXCL12-sfGFP
Plasmid#98961PurposeMammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCL12 (CXCL12 Human)
TagsSuperfolder GFPExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
E. coli groE operon
ExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A
Plasmid#100146PurposeExpresses CPT1ADepositorAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1-DUX4-CA
Plasmid#99281PurposeAll-in-one lentivector with tet-inducible DUX4 (codon sequences altered)DepositorInsertDUX4 (codon altered) (DUX4 Human)
UseLentiviralExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMGF182
Plasmid#97006PurposeExpresses GFP-CHMP7 in mammalian cells under a crippled promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHMP7 (CHMP7 Human)
TagsGFPExpressionMammalianPromoterCMV delta 5Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianMutationR188Q in FLucAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-VP6SA11
Plasmid#89166PurposeExpress Rotavirus SA11 VP6 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 VP6
ExpressionMammalianPromoterT7 promotorAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#84446PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.DepositorHas serviceAAV1, AAV5, and AAV8InsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_KRAS_p.G12D
Plasmid#81651PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-NSP4SA11
Plasmid#89171PurposeExpress Rotavirus SA11 NSP4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 NSP4
ExpressionMammalianPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GRB2-GFP
Plasmid#86873PurposeTo express GRB2 with a GFP tag at C terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRB2 (GRB2 Human)
TagsGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Pooled library#1000000100PurposeCRISPR gRNA knockout pooled library (version 1) for targeting the human genomeDepositorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Rtn4a
Plasmid#86683PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by