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LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
96971pMGF130S. pombe lem2 knockout cassette with hygro resistance gene
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96972pMGF157ish1-mCherry insertion cassette with Hygro resistance gene
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96996pMGF169ish1 mCherry insertion cassette with G418 resistance gene
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96997pMGF170ish1-mCherry insertion cassette with NAT resistance gene
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96998pMGF172ish1 mCherry insertion cassette with ura4+ gene
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96999pMGF173NLS-GFP-lacZ leu1 insertion vector
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97005pMGF196Expresses LEMD2-mCherry in mammalian cells under a crippled promoter
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97006pMGF182Expresses GFP-CHMP7 in mammalian cells under a crippled promoter
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