The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation.
Xu Y, Anderson DE, Ye Y
Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86857 | pVENUS-N1-Ubl4A | To express Ubl4A tagged with VENUS at C terminus. |
| 86858 | pcDNA5/FRT/TO-FNTA-FLAG | To express FNTA with a FLAG tag at C terminus. |
| 86859 | pVENUS-N1-FNTA | To express FNTA with a Venus tag at C terminus. |
| 86869 | pcDNA5/FRT/TO-FNTB-HA | To express FNTB with a HA tag at C terminus. |
| 86870 | pEGFP-C1-GFP-SF3B6-FLAG | To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively. |
| 86871 | pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG | To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively. |
| 86872 | pVENUS-N1-POLR2G-GFP | To express POLR2G (RPB7) tagged with VENUS at C terminus. |
| 86873 | pcDNA5/FRT/TO-GRB2-GFP | To express GRB2 with a GFP tag at C terminus. |
| 86874 | pcDNA5/FRT/TO-FLAG-MORF4L1(iso2) | To express MORF4L1 (iso2) with a FLAG tag at N-terminus. |
| 86875 | pcDNA5/FRT/TO-FLAG-CHK1 | To express CHK1 with a FLAG tag at N-terminus. |
| 86921 | pEGFP-C1-FLAG-GFP-Ubl4A | To express Ubl4A tagged with FLAG-EGFP at N- terminus. |
| 86922 | pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89) | To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus. |
| 86923 | pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157) | To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus. |
| 86924 | HUWE1-sgRNA-1 | To express sgRNA target HUWE1 gene |
| 86925 | HUWE1-sgRNA-2 | To express sgRNA target HUWE1 gene |
| 87202 | pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107) | To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus. |
| 87203 | pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130) | To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus. |