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Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning.
Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98942pcDNA3-CXCR4Mammalian expression plasmid for untagged human CXCR4
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98943pcDNA3-CCR5Mammalian expression plasmid for untagged human CCR5
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98944pCEP4-CXCR4Mammalian expression plasmid for untagged human CXCR4
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98945pCEP4-CCR5Mammalian expression plasmid for untagged human CCR5
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98946pCEP4-myc-CXCR4Mammalian expression plasmid for N-terminal myc-tagged human CXCR4
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98947pCEP4-FLAG-CXCR4Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4
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98948pCEP4-myc-CCR5Mammalian expression plasmid for N-terminal myc-tagged human CCR5
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98949pCEP4-FLAG-CCR5Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5
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98950pCEP4-HA-CCR5Mammalian expression plasmid for N-terminal HA-tagged human CCR5
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98951pCEP4-myc-CXCR4-W161YMammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y
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98952pCEP4-myc-CXCR4-W161HMammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H
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98953pCEP4-myc-CXCR4-H79QMammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q
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98954pCEP4-CCR5(R5gp120-Hi)Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi
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98957pCEP4-gp120(BaL)-CD4Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
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98961pcDNA3-CXCL12-sfGFPMammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12
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98962gRNA-CXCR4(90)Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2
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98963pCEP4-FLAG-CCR5-VNFLAG-tagged human CCR5 fused to N-terminus of split Venus
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98964pCEP4-FLAG-CXCR4-VNFLAG-tagged human CXCR4 fused to N-terminus of split Venus
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98965pCEP4-FLAG-GRM3-VNFLAG-tagged human mGluR3 fused to N-terminus of split Venus
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98966pCEP4-myc-CCR5-VCmyc-tagged human CCR5 fused to C-terminus of split Venus
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98967pCEP4-myc-CXCR4-VCmyc-tagged human CXCR4 fused to C-terminus of split Venus
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98968pCEP4-myc-GRM3-VCmyc-tagged human mGluR3 fused to C-terminus of split Venus
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