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pcDNA3-CXCL12-sfGFP
(Plasmid #98961)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98961 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5344
  • Total vector size (bp) 6379
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CXCL12
  • Alt name
    SDF-1
  • Alt name
    SDF1
  • Alt name
    SDF1alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1035
  • Mutation
    Full-length, wildtype
  • GenBank ID
    NM_199168.3
  • Entrez Gene
    CXCL12 (a.k.a. IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Superfolder GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-CXCL12-sfGFP was a gift from Erik Procko (Addgene plasmid # 98961 ; http://n2t.net/addgene:98961 ; RRID:Addgene_98961)
  • For your References section:

    Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E. J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343. 10.4049/jimmunol.1800343 PubMed 29678950