pBABE puro SDF-1 alpha
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12270||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5150
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSDF-1 alpha
Alt namestromal cell-derived factor 1
SpeciesH. sapiens (human)
Insert Size (bp)400
Entrez GeneCXCL12 (a.k.a. IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI/SalI (destroyed during cloning)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
A 0.4kb BamHI/XhoI fragment of the human SDF-1 alpha cDNA was cloned into the BamHI/SalI site of the pBABE-puro vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE puro SDF-1 alpha was a gift from Bob Weinberg (Addgene plasmid # 12270 ; http://n2t.net/addgene:12270 ; RRID:Addgene_12270)
For your References section:Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA. Cell. 2005 May 6. 121(3):335-48. 10.1016/j.cell.2005.02.034 PubMed 15882617