pBABE-puro HGF
(Plasmid #10901)

• Depositing Lab: Bob Weinberg
• Publication: Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HGF
• Alt name: hepatocyte growth factor
• Species: H. sapiens (human)
• Entrez Gene: HGF (a.k.a. DFNB39, F-TCF, HGFB, HPTA, SF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: G. Vande Woude
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry