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A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs.
Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R
Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117.
PubMed Article

Plasmids from Article

ID Plasmid  
100935LLP109 pEF1a-gRNA-GFP
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100936LLP185 pLVP-dCas9-DNMT3a V2
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100937LLP245 pEF1a-TALE-DNMT3a-Wt
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100938LLP249 pGK-dCas9-DNMT3a V3
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100939LLP250 pEF1a-TALE-SunTag
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100940LLP251 pEF1a-UNC5C-4-SunTag
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100941LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
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100942LLP338 pEF1a-DACH1-1-SunTag
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100943LLP339 pGK-dCas9-SunTag
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100944LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)
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100945LLP453 pEf1a-NLS-aGCN4-mCherry
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100957LLP457 pGK-dCas9-Suntag-BFP
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100958LLP469 pEF1a-mCherry-EMPTY-gRNA
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