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LLP185 pLVP-dCas9-DNMT3a V2
(Plasmid #100936)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100936 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVP
  • Backbone size w/o insert (bp) 6235
  • Total vector size (bp) 12934
  • Modifications to backbone
    Addgene Plasmid #41816 hCas9_D10A with PCR mutagenesis to introduce the H840A mutation to generate the catalytically dead Cas9 (dCas9) and ZF-598-DNMT3A plasmid (Gift from Pilar Blancafort) was used to PCR amplify DNMT3a catalytic domain
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9, DNMT3a catalytic domain
  • Species
    H. sapiens (human); S.pyogenes
  • Insert Size (bp)
    5157
  • Mutation
    D10A, H840A for dCas9
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, M.HsaIIIA, TBRS)
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • 3xTy1 (C terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP185 pLVP-dCas9-DNMT3a V2 was a gift from Ryan Lister (Addgene plasmid # 100936 ; http://n2t.net/addgene:100936 ; RRID:Addgene_100936)
  • For your References section:

    A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613