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PurposeThe plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1
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Depositing Lab
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Sequence Information
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Sequences (4) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDNMT3A1
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SpeciesH. sapiens (human)
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Insert Size (bp)2736
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Entrez GeneDNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
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Tag
/ Fusion Protein
- scFV-sfGFP (N terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
scFv-sfGFP-DNMT3A1 was a gift from Margaret Goodell (Addgene plasmid # 102278 ; http://n2t.net/addgene:102278 ; RRID:Addgene_102278) -
For your References section:
DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A. Huang YH, Su J, Lei Y, Brunetti L, Gundry MC, Zhang X, Jeong M, Li W, Goodell MA. Genome Biol. 2017 Sep 18;18(1):176. doi: 10.1186/s13059-017-1306-z. 10.1186/s13059-017-1306-z [pii] PubMed 28923089
