pCDNA3 Flag BimL
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24233||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBim L
SpeciesH. sapiens (human)
Insert Size (bp)458
Entrez GeneBCL2L11 (a.k.a. BAM, BIM, BOD)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3 Flag BimL was a gift from Roger Davis (Addgene plasmid # 24233 ; http://n2t.net/addgene:24233 ; RRID:Addgene_24233)
For your References section:JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei K, Davis RJ. Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. 10.1073/pnas.0438011100 PubMed 12591950