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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-Cs

Plasmid

#183171

Purpose

Construct for transient expression of cytoskeleton-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Cs

Tags

363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 alpha

Plasmid

#20351

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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BAP1-wt (siRNA resistant)

Plasmid

#108439

Purpose

Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

Promoter

CMV

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEQ176

Plasmid

#83943

Purpose

express beta-galactosidase in eukaryotic cells

Depositor

Adam Geballe

Article

Schleiss et al J Virol. 1991 Dec;65(12):6782-9.

Insert

CMV enhancer/promoter

Tags

beta-galactosidase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAS162

Plasmid

#194485

Purpose

synGR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synGR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pS34.EF1α<GFP-2A-Cas9-RC

Plasmid

#207404

Purpose

Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

Cas9-RC

Use

CRISPR and Synthetic Biology

Tags

GFP-2A

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa3

Plasmid

#33005

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ203 (pMDC32-Ubi1)

Plasmid

#86207

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance marker

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Promoter

Maize ubiquitin 1 (ZmUbi1)

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEE393

Plasmid

#149283

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-DTAG-CTERM-GFP

Plasmid

#185770

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNPK-NLS-Vika

Plasmid

#79969

Purpose

expresses human codon-optimized Vika-recombinase in mammalian cells

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

human codon-optimized Vika sequence, NLS tagged

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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PX551

Plasmid

#60957

Purpose

pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Insert

SpCas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-ARF19

Plasmid

#83846

Purpose

Gateway donor vector for ARF19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

ARF19 (ARF19 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_OCA2

Plasmid

#132253

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

OCA2 (OCA2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLX317-MBNL2

Plasmid

#115444

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL2 (MBNL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-Isce1-eno2:egfp

Plasmid

#61394

Purpose

Expresses GFP in neurons of transgenic zebrafish

Depositor

Edward Burton

Article

Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

Insert

eno2 regulatory element

Use

Zebrafish expression

Promoter

eno2

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

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