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(Plasmid #44168)


Item Catalog # Description Quantity Price (USD)
Plasmid 44168 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 10249
  • Modifications to backbone
    Removed Neo gene from pcDNA3.1
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    May grow better in E. coli strain HB101.
  • Copy number
    High Copy


  • Gene/Insert name
    Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
  • Species
    Genes from Wyoming strain EIAV
  • Insert Size (bp)
  • Mutation
    A131T mutation in gag; Deletion of viral LTR sequences, deletion of viral packaging signal, deletion of first exon of tat gene, extensive deletion of env gene
  • GenBank ID
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Frederick Fuller (North Carolina State University, Raleigh, NC, USA).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A131T mutation in gag compared to GenBank reference sequence M87581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV53D was a gift from John Olsen (Addgene plasmid # 44168 ; ; RRID:Addgene_44168)
  • For your References section:

    High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. Patel M, Giddings AM, Sechelski J, Olsen JC. J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. 10.1002/jgm.2695 PubMed 23319179