273,069 results

1. NSUN5

   Plasmid

   #25491

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NSUN5 (NSUN5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. NSUN5

   Plasmid

   #25491

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NSUN5 (NSUN5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. nuclear NAD+ biosensor

   Plasmid

   #186789

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_HAPLN1_WT

   Plasmid

   #81757

   Purpose

   Gateway Donor vector containing HAPLN1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HAPLN1 (HAPLN1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_HAPLN1_WT

   Plasmid

   #81757

   Purpose

   Gateway Donor vector containing HAPLN1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HAPLN1 (HAPLN1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pISV-CRISPR/Cas9PGmUbi

   Plasmid

   #209189

   Purpose

   Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

   Depositor

   Christian Staehelin

   Inserts

   Cas9
   DsRed(deltaB)
   

   Tags

   NLS

   Expression

   Plant

   Mutation

   missing BsaI site and plant-codon optimized with …

   Promoter

   CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1 GFP B55 alpha shRNA 3-1

   Plasmid

   #10679

   Depositor

   William Hahn

   Article

   Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

   Insert

   PP2A B55 alpha shRNA (PPP2R2A Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pMKO.1 GFP B55 alpha shRNA 3-1

    Plasmid

    #10679

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B55 alpha shRNA (PPP2R2A Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LEGO-AP1x3-GM55

    Plasmid

    #217414

    Purpose

    Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP15457 - pAAV-AiE0441h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5457)

    Plasmid

    #224198

    Purpose

    AiE0441h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-VIP [L108/92R]

    Plasmid

    #177453

    Purpose

    Mammalian Expression Plasmid of anti-VIP (Human). Derived from hybridoma L108/92.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody (VIP Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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