164,778 results

1. tdTomato-β-actinCDS3

   Plasmid

   #183715

   Purpose

   tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXalb_IC_Cascade

   Plasmid

   #178765

   Purpose

   Expression of X. albilineans type I-C Cascade genes

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   X. albilineans type I-C Cascade genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221_TMEM104

   Plasmid

   #132262

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   TMEM104 (TMEM104 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. EGFP-Tubulin-6

   Plasmid

   #56450

   Purpose

   Localization: Microtubules, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. gag-TadCBEd

   Plasmid

   #225144

   Purpose

   Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-TadCBEd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FAB(ER-mito)

   Plasmid

   #214427

   Purpose

   Detection of ER-mito contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FRB-V5
   

   Use

   Tags

   CFAST10-Halo and RspAN-FRB-V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Spike-VLP

   Plasmid

   #173922

   Purpose

   Expresses virus-like particles based upon the original SARS-Cov-2

   Depositor

   Andreas Stuermer

   Inserts

   SARS-2 Spike Protein (S Human, SARS-COV-2, Synthetic)
   SARS-Cov-2 E protein (E Human, SARS-COV-2, Synthetic)
   SARS-Cov-2 M Protein (M Human, SARS-COV-2, Synthetic)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCAGEN

   Plasmid

   #11160

   Purpose

   Mammalian expression vector for cloning and expressing your gene under the CAG promoter

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   modified multiple cloning sites
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-SSTR3-N-17

   Plasmid

   #55135

   Purpose

   Localization: Cilium, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   SSTR3
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4 myc PGC-1 alpha

    Plasmid

    #10974

    Purpose

    Mammalian expression of PGC-1a with myc and his tags

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    PGC-1 alpha (PPARGC1A Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral Prep

    #20298-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

12. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

13. tdTomato-C1

    Plasmid

    #54653

    Purpose

    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid

    #220800

    Purpose

    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

    Depositor

    Elizabeth Kirby

    Article

    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

    Insert

    Mars1 (Mars1 Mouse)
    

    Use

    Lentiviral

    Tags

    2xFLAG and T2A-mCherry

    Expression

    Mutation

    mutation in MetRS to change aa 274 from L to G

    Promoter

    EF1a

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TL026_AAV_hSyn:SEP-GluA1

    Plasmid

    #192519

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    AAV and Cre/Lox

    Tags

    super ecliptic phluorin

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP/KATNA1

    Plasmid

    #89316

    Purpose

    Expression of rat GFP/Katanin A subunit in mammalian cells

    Depositor

    Peter Baas

    Article

    Karabay et al J Neurosci. 2004 Jun 23;24(25):5778-88.

    Insert

    KATNA1 (Katna1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. spNW30

    Plasmid

    #173485

    Purpose

    Expresses spNW30 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW30
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pScarlet-N1

    Plasmid

    #128060

    Purpose

    Mammalian expression vector for C terminal mScarlet-I fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge