pFUSEss-CHIg-mG1_M18
(Plasmid #82357)

• Purpose: Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.
• Depositing Lab: Joanna Bereta
• Publication: Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.1038/srep30938.

Backbone

• Vector backbone: pFUSEss-CHIg-mG1
• Backbone manufacturer: Invivogen
• Backbone size w/o insert (bp): 4492
• Total vector size (bp): 4829
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Top10 or DH5alpha could be selected on LB LOW SALT with 35-50 micrograms/ml Zeocin.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse immunoglobulin heavy chain IgG1 isotype
• Alt name: IgG1 HC
• Species: M. musculus (mouse)
• Insert Size (bp): 367
• Mutation: none
• GenBank ID:
• Entrez Gene: Ighg1 (a.k.a. IgG1, Igh-4, VH7183)
• Promoter: hEF1-HTLV prom
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: AfeI (not destroyed)
• 5′ sequencing primer: GCGAAGGAGGGCCACCATG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry