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Ivan Yudushkin Lab Plasmids


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Title Authors Publication
PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Ebner M, Lucic I, Leonard TA, Yudushkin I Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028.
Localization of mTORC2 activity inside cells. Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
72899pHR-FRB:Akt2Serine/threonine protein kinase Bβ (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Recruitable Akt2 (LocaTOR2) pHR' AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
72900pHR-FKBP:mCherry-KRas4Bc30GTPase K-Ras (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Membrane-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72901pHR-FKBP:mCherry-Rab5aRab5a (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Early endosome-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72902pHR-FKBP:mCherry-Rab11aRab11a (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Recycling endosome-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72903pHR-FKBP:mCherry-Rab7aRab7a (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Late endosome-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72904pHR-TcRb:mCherry-FKBPT cell receptor β chain (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. ER-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72905pHR-FKBP:mCherry-BclXLc33Bl2-like protein 1, isoform Bcl-X(L) (fragment) (Homo sapiens)Mammalian Expression, LentiviralLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Outer mitochondrial membrane-targeted mCherry-tagged recruiter construct pHR' Add to Cart
72906C1-FKBP(F36V):mCherry-Akt2(K14A)RAC-beta serine/threonine-protein kinase (Homo sapiens)Mammalian ExpressionLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. Recruitable Akt2(K14A) mutant C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
72907N1-mSin1.1-GFPmSin1.1 (Homo sapiens)Mammalian ExpressionLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. GFP-tagged mSin1 (isoform 1) pEGFP-N1 MAPKAP1 JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
72908N1-mSin1.2-GFPTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (Homo sapiens)Mammalian ExpressionLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. GFP-tagged mSin1 (isoform 2) N1 MAPKAP1 JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
72909N1-mSin1.5-GFPTarget of rapamycin complex 2 subunit MAPKAP1, isoform 5 (Homo sapiens)Mammalian ExpressionLocalization of mTORC2 activity inside cells. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. GFP-tagged mSin1 (isoform 5) N1 MAPKAP1 JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
86561pFBD-Akt1(WT)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses human full-length Akt1(WT) pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86562pFBD-Akt1(K14A)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses human full-length Akt1(K14A) mutant pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86563pFBD-Akt1(E17K)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses human full-length Akt1(E17K) mutant pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86576pFBD-Akt13C(WT)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses cleavable human full-length Akt1(WT) pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86577pFBD-Akt13C(S473D)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses cleavable human full-length Akt1(S473D) phosphomimetic pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86578pFBD-Akt1(T308A/S473A)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses human full-length Akt1(T308A/S473A) phosphomutant pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86591pFBD-Btk(PH)Akt1(KD)PH(Btk)-KD(Akt1) fusion (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1 pFastBac Dual Add to Cart
86592pFBD-Akt1(D323A/D325A)Akt1 (Homo sapiens)Insect ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses human full-length Akt1(D323A/D325A) mutant pFastBac Dual AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86593pEGFP-Akt2Akt2 (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged human Akt2(WT) pEGFP-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86594pTagBFP-Akt1Akt1 (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses TagBFP-tagged human Akt1(WT) pTagBFP AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86595pEGFP-Akt2(K14A/R25E)Akt2(K14A/R25E)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant pEGFP-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86596pmCherry-PH(Akt2)Akt2 PH domain (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged PH(WT) domain of human Akt2 pmCherry-C1 Add to Cart
86597pFKBPmCherry-Akt2(K14A)FKBB-mCherry-Akt2(K14A)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant pmCherry-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86598pFKBPmCherry-Akt2(KD)FKBP-mCherry-Akt2(KD)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT) pmCherry-C1 Add to Cart
86599pEGFP-PH1(WT)Akt1 PH domain (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged PH(WT) domain of human Akt1 pEGFP-C1 Add to Cart
86600pmCherry-PH1(K14A)K14A mutant of Akt1 PH domain (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged PH(K14A) domain of human Akt1 pmCherry-C1 Add to Cart
86623pmCherry-Akt2(WT)Akt2 (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged human Akt2(WT) pmCherry-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86624pmCherry-Akt2(K14A)Akt2(K14A)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant pmCherry-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86625pEGFP-Akt2(T309A)Akt2(T309A) (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged human Akt2(T309A) phosphomutant pEGFP-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86626pHaloTag-PSHaloTag-PS(RA)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses Akt pseudosubstrate tagged with a HaloTag pHaloTag-C1 Add to Cart
86627pHaloTag-PS(RA)HaloTag-PS(RA)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTag pHaloTag-C1 Add to Cart
86628pmCherry-PSAkt pseudosubstrate (VELDPEFEPRARERAYAFGH)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses Akt pseudosubstrate tagged with mCherry pmCherry-C1 Add to Cart
86629pHaloTag-EGFPHaloTagMammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses HaloTag-eGFP tandem (cytosolic) pEGFP-N1 Add to Cart
86630pEGFP-Akt1(D323A/D325A)Akt1(D323A/D325A) (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged human Akt1(D323A/D325A) mutant pEGFP-C1 AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86631pmCherry-Akt1(WT)Akt1 (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged human Akt1(WT) pmCherry-C1 AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86632pEGFP-Akt2(K14A/R25E)-Halo-PSAkt2(K14A/R25E)-Halo-PSMammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem) pmCherry-C1 AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA Add to Cart
86633pHR-FKBP:mCherry-KRas4Bc30FKBP:mCherry-KRas4Bc30Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged membrane recruiter construct pHR Add to Cart
86634pHR-FRB:Akt1(D274A)FRB-Akt1(D274A) (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant pHR AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86635pEGFP-PH(Btk)Btk PH domain (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-taggd PH domain of Btk pEGFP-C1 Add to Cart
86636pmCherry-PH(Btk)KD(Akt1)PH(Btk)KD(Akt1)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1 pmCherry-C1 Add to Cart
86637pEGFP-Akt1(WT)Akt1 (Homo sapiens)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses eGFP-tagged human Akt1(WT) pEGFP-C1 AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
86638pHR-FKBP:mCherry-Rab7aFKBP-mCherry-Rab7aMammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-tagged Rab7a pHR Add to Cart
86639pmCherry-EGFPmCherry-EGFP (Other)Mammalian ExpressionPI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. Expresses mCherry-eGFP tandem (cytoplasmic) pEGFP-N1 Add to Cart