Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #105921)


Item Catalog # Description Quantity Price (USD)
Plasmid 105921 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
AAV2 105921-AAV2 100 µL at titer ≥ 7×10¹² vg/mL and Plasmid. $405

* Login to view industry pricing.


  • Vector backbone
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Mutation
    codon optimized
  • Promoter CBh

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCBhProF2 (5-agggtttaagggatggttgg)
  • 3′ sequencing primer pCDH-rev (5-GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    IRES-Multiple cloning site
  • Alt name
    MCS is FseI-PacI-SpeI-BamHI-XhoI-StuI-MluI-NheI-BmtI-AsiSI-AscI
  • Promoter same CBh promoter as mKate2

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer IRES-F (5-TGGCTCTCCTCAAGCGTATT)
  • 3′ sequencing primer WPRE-R (5-CATAGCGTAAAAGGAGCAACA)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entire transgene cassette can be removed with NotI digest; CBh promoter can be removed by XbaI/AgeI digest; mKate2 can be removed with SaI/EcoRI digest; IRES can be removed by EcoRI/FseI digest

Information for AAV2 (Catalog # 105921-AAV2) ( Back to top )


Ready-to-use AAV2 particles produced from pAAV-CBh-mKate2-IRES-MCS (WT-WT) (#105921). In addition to the viral particles, you will also receive purified pAAV-CBh-mKate2-IRES-MCS (WT-WT) plasmid DNA.

Control mKate2 expression AAV with two wild-type ITR sequences. These AAV preparations are suitable purity for injection into animals.


  • Volume 100 µL
  • Titer ≥ 7×10¹² vg/mL
  • Pricing $375 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
  • Buffer PBS + 0.001% Pluronic F-68 + 200 mM NaCl
  • Serotype AAV2
  • Purification Iodixanol gradient ultracentrifugation
  • Reporter Gene mKate2


Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CBh-mKate2-IRES-MCS (WT-WT) was a gift from Marcella Patrick (Addgene plasmid # 105921 ; ; RRID:Addgene_105921)

    For viral preps, please replace (Addgene plasmid # 105921) in the above sentence with: (Addgene viral prep # 105921-AAV2)