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Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA
Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. PubMed Article

Plasmids from Article

ID Plasmid Purpose
85421pHR_PGK_LaG16_2_synNotch_Gal4VP64Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch
85422pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch
85423pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64Lentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic Notch
85424pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch
85425pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch
85426pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression
85427pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression
85428pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression
85429pHR_Gal4UAS_humanIL-2_T2A_MIP1α_PGK_mCherryLentiviral vector for Gal4 inducible human IL-2/MIP1a with constitutive mCherry expression
85430pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression
85431pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherryLentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression
85432pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression
85433pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression
85434pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherryLentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression
85436pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherryLentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression
85437pHR_Gal4UAS_Blinatumomab_PGK_mCherry[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression
85438pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFPLentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Antibodies from Article