PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85421||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepHR SIN
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameLaG16_2 GFP nanobody synNotch Gal4VP64
- Promoter PGK
- Cloning method Gateway Cloning
- 5′ sequencing primer gcttcaaaagcgcacgtct (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR_PGK_LaG16_2_synNotch_Gal4VP64 was a gift from Wendell Lim (Addgene plasmid # 85421 ; http://n2t.net/addgene:85421 ; RRID:Addgene_85421)
For your References section:Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. 10.1016/j.cell.2016.09.011 PubMed 27693353