Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCW57.1-FOXM1b
(Plasmid #68811)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68811 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCW57.1
  • Backbone manufacturer
    Broad Insitute
  • Backbone size w/o insert (bp) 7667
  • Total vector size (bp) 10061
  • Vector type
    Mammalian Expression, Lentiviral ; Doxycycline inducible
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FOXM1b
  • Alt name
    Forkhead Box M1b
  • Alt name
    FOXM1
  • Alt name
    Forkhead Box M1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2407
  • GenBank ID
    NM_202003.2
  • Entrez Gene
    FOXM1 (a.k.a. FKHL16, FOXM1A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT)
  • Promoter TRE promoter, Tet ON
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
  • 3′ sequencing primer O.PGK1b-R (5'-GAACGGACGTGAAGAATGTG-3')
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The human FOXM1b open reading frame was subcloned from Origene FOXM1b cDNA construct (Cat SC128214 ) into Origene (Cat PS100013) pCMV6-AN-HA then finally subcloned into pCW57.1.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Note: The plasmid is derived from a FOXM1b cDNA clone from Origene which contains a SNP which causes a serine to proline amino acid residue substitution. This residue change is a natural variant and does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-FOXM1b was a gift from Adam Karpf (Addgene plasmid # 68811 ; http://n2t.net/addgene:68811 ; RRID:Addgene_68811)
  • For your References section:

    Genetic determinants of FOXM1 overexpression in epithelial ovarian cancer and functional contribution to cell cycle progression. Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, Vanderhyden BC, Odunsi K, Karpf AR. Oncotarget. 2015 Jul 16. 4546 [pii] PubMed 26243836