164,499 results

1. pGEX-6P-1_WT RAD23B

   Plasmid

   #201457

   Purpose

   Expresses human RAD23B with an N-terminal GST tag in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   tac promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-dLight1.5

   Plasmid

   #111058

   Purpose

   Expresses dLight1.5 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.5
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT85_PS-mEos3.2_GentaR

   Plasmid

   #173895

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Spiralin

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPICZaa-STE2-cohis

   Plasmid

   #133433

   Purpose

   Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C59S/C252A, Codon optimized for P. pastoris, and …

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMaCTag-P29

   Plasmid

   #120040

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF0335

   Plasmid

   #144358

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF91 (ZNF91 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pN1-PinkyCaMP

   Plasmid

   #232861

   Purpose

   A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPGK-T7/2-CD44s (fl)

   Plasmid

   #137812

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44s (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length, I186T and I253V on CD44

   Promoter

   PGK

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HTR1A-Tango

   Plasmid

   #66404

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR1A (HTR1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOT_7-lenti-EFS-SS1-BBz-2A-puro

    Plasmid

    #217990

    Purpose

    Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Xue et al bioRxiv 2024.11.27.625752

    Insert

    SS1-BBz CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RFP-LC3B

    Plasmid

    #200943

    Purpose

    RFP fused with LC3B for reporting LC3B lipidation

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBWB162

    Plasmid

    #140634

    Purpose

    pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUGW-hGtACR2-EYFP

    Plasmid

    #67877

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M77 pIRESpuro-GLUE-hDsh3

    Plasmid

    #15102

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    Dsh3 (DVL3 Human)
    

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge