CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T
Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x. PubMed Article
Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T
Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134919 | pCAG-All-in-one-hCascade | Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein. |
| 134920 | pPB-CAG-hCas3 | Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted. |
| 134921 | pBS-U6-crRNA-empty | Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade. |
| 134922 | pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA | Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein. |
| 134923 | pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA | Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein. |
| 134924 | pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA | Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein. |
| 134925 | pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA | Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein. |
| 204619 | pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD) | Expression vector of Cascade and Cas3. Puromycin selectable. |