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Addgene
A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells.
Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. PubMed Article

These plasmids are included in The Mammalian Toolkit. Please see the following article page for additional plasmids cited in this publication.

Plasmids from Article

ID Plasmid Purpose
123671MTK1_008Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123672MTK1_009Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123673MTK1_010Encodes ConL2 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123674MTK1_011Encodes ConL3 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123675MTK1_012Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123676MTK1_013Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123677MTK1_014Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system
123678MTK1_015Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system
123679MTK1_016Encodes ConL6 as a Type 1 part to be used in the MTK system
123680MTK1_017Encodes ConL7 as a Type 1 part to be used in the MTK system
123681MTK1_018Encodes ConL8 as a Type 1 part to be used in the MTK system
123682MTK1_019Encodes ConL6 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123683MTK1_020Encodes ConL7 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
123684MTK1_022Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system
123685MTK1_023Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system
123686MTK1_024Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system
123687MTK1_025Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system
123688MTK1_026Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system
123689MTK1_027Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system
123690MTK1_028Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system
123691MTK1_029Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system
123692MTK1_030Encodes ConL1 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
123693MTK1_031Encodes ConL2 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
123694MTK1_032Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
123695MTK1_033Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
123696MTK1_034Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
123697MTK2_001Encodes pTRE as a Type 2 part to be used in the MTK system
123698MTK2_003Encodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK system
123699MTK2_004Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system
123700MTK2_005Encodes pCAG as a Type 2 part to be used in the MTK system
123701MTK2_006Encodes pSV40 as a Type 2 part to be used in the MTK system
123702MTK2_007Encodes human pEF1alpha as a Type 2 part to be used in the MTK system
123703MTK2_008Encodes human pUbc as a Type 2 part to be used in the MTK system
123704MTK2_012Encodes a spacer as a Type 2 part to be used in the MTK system
123705MTK2_013Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system
123706MTK2_014Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system
123707MTK2_015Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK sytem
123708MTK2_016Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123709MTK2_017Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123710MTK2_018Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123711MTK2_019Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123712MTK2_020Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123713MTK2_021Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123714MTK2_022Encodes truncated pCMV as a Type 2 part to be used in the MTK system
123715MTK2_023Encodes pCMV as a Type 2 part to be used in the MTK system
123716MTK2_024Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system
123717MTK2_025Encodes crippled human pEF1alpha as a Type 2 part to be used in the MTK system
123718MTK2_026Encodes crippled pSFFV as a Type 2 part to be used in the MTK system
123719MTK3_002Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system
123720MTK3_004Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system
123721MTK3_005Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system
123722MTK3_006Encodes human histone H2B as a Type 3 part to be used in the MTK system
123723MTK3_008Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system
123724MTK3_009Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system
123725MTK3_010Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system
123726MTK3_011Encodes HA-tagged PIF6 fused to Tet Repressor protein as a Type 3 part to be used in the MTK system
123727MTK3_012Encodes HA-tagged PYLcs fused to VP16 activator domain as a Type 3 part to be used in the MTK system
123728MTK3_013Encodes Flag-tagged ABIcs fused to GAL4 DNA binding domain as a Type 3 part to be used in the MTK system
123729MTK3_014Encodes Gq-coupled hM3D as a Type 3 part to be used in the MTK system
123730MTK3_015Encodes Gi-coupled hM4D as a Type 3 part to be used in the MTK system
123731MTK3_016Encodes Gs-coupled rM3D as a Type 3 part to be used in the MTK system
123732MTK3_017Encodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK system
123733MTK3_018Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system
123734MTK3_019Encodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK system
123735MTK3_020Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system
123736MTK3_021Encodes ERK Translocation Reporter (ERK KTR) as a Type 3 part to be used in the MTK system
123741MTK3_026Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system
123742MTK3_027Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system
123743MTK3_029Encodes PKA SPARK, a PKA activity reporter, as a Type 3 part to be used in the MTK system
123744MTK3_030Encodes ERK SPARK, a ERK activity reporter, as a Type 3 part to be used in the MTK system
123745MTK3_031Encodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as a Type 3 part to be used in the MTK system
123746MTK3_032Encodes JNK Translocation Reporter (JNK KTR) fused to mRuby2 as a Type 3 part to be used in the MTK system
123747MTK3_033Encodes Gibberellin Receptor fused to VPR activation domain as a Type 3 part to be used in the MTK system
123748MTK3_034Encodes Absisic acid Receptor fused to VPR activation domain as a Type 3 part to be used in the MTK system
123749MTK3_035Encodes human E-Cadherin as a Type 3 part to be used in the MTK system
123750MTK3_036Encodes mScarlet as a Type 3 part to be used in the MTK system
123751MTK3_037Encodes cAMPr, a cAMP reporter, as a Type 3 part to be used in the MTK system
123752MTK3_038Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system
123753MTK3_039Encodes mouse RelA as a Type 3 part to be used in the MTK system
123754MTK3a_001Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK
123755MTK3a_002Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system
123756MTK3a_003Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system
123757MTK3a_004Encodes Golgi Targeting Sequence as a Type 3a part to be used in the MTK system
123758MTK3a_005Encodes Mitochondria Targeting Sequence as a Type 3a part to be used in the MTK system
123759MTK3a_006Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be used in the MTK system
123760MTK3a_007Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system
123761MTK3a_008Encodes ERK Translocation Reporter (ERK KTR) as a Type 3a part to be used in the MTK system
123762MTK3a_009Encodes BxBI attP site as a Type 3a part to be used in the MTK system
123763MTK3a_010Encodes Phi31 attP site as a Type 3a part to be used in the MTK system
123764MTK3a_011Encodes EGFP as a Type 3a part to be used in the MTK system
123765MTK3a_013Encodes msGFP as a Type 3a part to be used in the MTK system
123766MTK3a_014Encodes Kruppel Associated Box repression domain as a Type 3a part to be used in the MTK system
123767MTK3a_015Encodes VPR activation domain as a Type 3a part to be used in the MTK system
123768MTK3a_016Encodes Absisic acid insensitive 1 as a Type 3a part to be used in the MTK system
123769MTK3a_017Encodes Gibberellin Insensitive as a Type 3a part to be used in the MTK system
123770MTK3a_018Encodes human histone H2B as a Type 3a part to be used in the MTK system
123771MTK3a_019Encodes Absisic acid receptor as a Type 3a part to be used in the MTK system
123772MTK3a_020Encodes Gibberellin Receptor as a Type 3a part to be used in the MTK system
123773MTK3a_021Encodes HELB-CDK2 Translocation Reporter as a Type 3a part to be used in the MTK system
123774MTK3a_022Encodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK system
123775MTK3a_023Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system
123776MTK3a_024Encodes spCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
123777MTK3a_025Encodes dspCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
123778MTK3a_026Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
123779MTK3a_027Encodes tagBFP as a Type 3a part to be used in the MTK system
123782MTK3b_001Encodes human beta actin as a Type 3b part to be used in the MTK system
123783MTK3b_002Encodes human alpha tubulin 1 as a Type 3b part to be used in the MTK system
123784MTK3b_003Encodes picornavirus peptide T2A element as a Type 3b part to be used in the MTK system
123785MTK3b_004Encodes mAzamiGreen as a Type 3b part to be used in the MTK system
123786MTK3b_005Encodes iRFP670 as a Type 3b part to be used in the MTK system
123787MTK3b_006Encodes tagBFP as a Type 3b part to be used in the MTK system
123788MTK3b_007Encodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 3b part to be used in the MTK system
123789MTK3b_008Encodes EBFP2 as a Type 3b part to be used in the MTK system
123791MTK3b_010Encodes mScarlet as a Type 3b part to be used in the MTK system
123792MTK3b_011Encodes iRFP713 as a Type 3b part to be used in the MTK system
123793MTK3b_012Encodes hph (hygromycin B phosphotransferase) as a Type 3b part to be used in the MTK system
123794MTK3b_013Encodes Flamindo2,a cAMP reporter, as a Type 3 part to be used in the MTK system
123795MTK3b_014Encodes picornavirus peptide P2A element as a Type 3b part to be used in the MTK system
123796MTK3b_015Encodes rat Connexin 26 as a Type 3b part to be used in the MTK system
123797MTK3b_016Encodes human Connexin 30 as a Type 3b part to be used in the MTK system
123798MTK3b_017Encodes rat Connexin 43 as a Type 3b part to be used in the MTK system
123799MTK3b_018Encodes dspCAS9 as a Type 3b part to be used in the MTK system
123800MTK3b_019Encodes dsaCAS9 as a Type 3b part to be used in the MTK system
123801MTK3b_020Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system
123802MTK3b_021Encodes VPR activation domain as a Type 3b part to be used in the MTK system
123803MTK3b_022Encodes Kruppel Associated Box repression domain as a Type 3b part to be used in the MTK system
123804MTK3b_024Encodes human NFATC2 as a Type 3b part to be used in the MTK system
123805MTK3b_027Encodes human TP53 as a Type 3b part to be used in the MTK system
123806MTK3b_028Encodes Venus as a Type 3b part to be used in the MTK system
123807MTK3b_029Encodes Tet-On 3G transactivator protein as a Type 3b part to be used in the MTK system
123808MTK3b_030Encodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system
123809MTK3b_031Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system
123810MTK4_001Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system
123811MTK4_002Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system
123812MTK4_003Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system
123813MTK4_004Encodes spacer sequence as a Type 4 part to be used in the MTK system
123814MTK4_006Encodes spacer sequence (multicistronic) as a Type 4 part to be used in the MTK system
123815MTK4a_001Encodes plasma membrane localization signal from Lyn kinase as a Type 4a part to be used in the MTK
123816MTK4a_002Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system
123817MTK4a_003Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system
123819MTK4a_005Encodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK system
123820MTK4a_006Encodes ER Targeting Sequence (N Terminal) as a Type 4a part to be used in the MTK system
123821MTK4a_007Encodes Peroxisome Targeting Sequence as a Type 4a part to be used in the MTK system
123822MTK4a_008Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system
123823MTK4a_010Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system
123824MTK4a_011Encodes a nuclear localized iRFP670 coexpression cassette as a Type 4a part to be used in the MTK system
123825MTK4a_012Encodes mScarlet as a Type 4a part to be used in the MTK system
123826MTK4a_013Encodes a nuclear localized iRFP713 coexpression cassette as a Type 4a part to be used in the MTK system
123827MTK4a_014Encodes a nuclear localized mAzamiGreen coexpression cassette as a Type 4a part to be used in the MTK system
123828MTK4a_015Encodes msfGFP as a Type 4a part to be used in the MTK system
123829MTK4a_016Encodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK system
123830MTK4a_017Encodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK system
123831MTK4a_018Encodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK system
123832MTK4a_019Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system
123833MTK4a_020Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system
123834MTK4a_021Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system
123835MTK4a_023Encodes Gibberellin Insensitive as a Type 4a part to be used in the MTK system
123836MTK4a_024Encodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system
123838MTK4a_026Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system
123839MTK4a_027Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system
123840MTK4b_001Encodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK system
123841MTK4b_002Encodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK system
123842MTK4b_003Encodes rabbit beta-globin polyadenylation signal as a Type 4b part to be used in the MTK system
123843MTK4b_004Encodes spacer sequence as a Type 4b part to be used in the MTK system
123844MTK4b_006Encodes spacer sequence (multicistronic) as a Type 4b part to be used in the MTK system
123852MTK5_008Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system
123853MTK5_009Encodes ConR6 as a Type 5 part to be used in the MTK system
123854MTK5_010Encodes ConR7 as a Type 5 part to be used in the MTK system
123855MTK5_011Encodes ConR8 as a Type 5 part to be used in the MTK system
123856MTK5_012Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system
123857MTK6_003Encodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK system
123858MTK6_004Encodes tagBFP, thosea asigna virus 2A (T2A) and Blasticidin resistance as a multicistronic cassette to be used in the MTK system
123859MTK6_005Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette to be used in the MTK system
123860MTK6_006Encodes a spacer cassette as a type 6 part to be used in the MTK system
123861MTK6_007Encodes the attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK system
123862MTK6_008Encodes the attB BxBI transposon recognition site cassette as a type 6 part to be used in the MTK system
123863MTK6_009Encodes pCMV-Hygromycin resistance cassette as a type 6 part to be used in the MTK system
123864MTK6_010Encodes FRT recognition site cassette as a type 6 part to be used in the MTK system
123865MTK6_011Encodes the inverted attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK system
123866MTK6_012Encodes the inverted attB BxBI transposon recognition site cassette as a type 6 part to be used in the MTK system
123867MTK6_013Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system
123868MTK6_014Encodes WPRE expressing tagBFP T2A Blasticidin resistance cassette without a terminator to be used in the MTK system
123869MTK6_015Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system
123870MTK7_001Encodes the 3' PiggyBac transposon recognition site cassette as a type 7 part to be used in the MTK system
123871MTK7_002Encodes the 5' 500 bp hRosa26 locus homology arm as a type 7 part to be used in the MTK system
123872MTK7_004Encodes the 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system
123873MTK7_005Encodes the 3' AAV ITR cassette as a type 7 part to be used in the MTK system
123874MTK7_006Encodes the FRT cassette as a type 7 part to be used in the MTK system
123875MTK7_007Encodes the promoter-less Blasticidin cassette as a type 7 part to be used in the MTK system
123876MTK7_009Encodes the 5' 500 bp cRosa26 locus homology arm as a type 7 part to be used in the MTK system
123877MTK7_010Encodes promoter-less tagBFP T2A Blasticidin resistance cassette to be used in the MTK system
123878MTK7_011Encodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK system
123879MTK7_012Encodes the 5' 500 bp hCYBYL locus homology arm as a type 7 part to be used in the MTK system
123880MTK7_013Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system
123881MTK7_014Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system
123884MTK8_004Encodes the F1 Ampicillin resistance cassette as a type 8 part to be used in the MTK system
123885MTK8_005Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system
123888MTK8a_004Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system
123889MTK8a_005Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system
123890MTK8b_001Encodes the 5' PiggyBac transposon recognition site cassette as a type 8b part to be used in the MTK system
123891MTK8b_002Encodes the 3' 500 bp hRosa26 locus homology arm as a type 8b part to be used in the MTK system
123892MTK8b_004Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system
123893MTK8b_005Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system
123894MTK8b_006Encodes the LoxP recognition site as a type 8b part to be used in the MTK system
123895MTK8b_008Encodes the 3' 500 bp cRosa26 locus homology arm as a type 8b part to be used in the MTK system
123896MTK8b_009Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system
123897MTK8b_010Encodes the 3' 500 bp hCLYBL locus homology arm as a type 8b part to be used in the MTK system
123898MTK8b_011Encodes the 3' 500 bp hAAVS1 locus homology arm as a type 8b part to be used in the MTK system
123900MTK234_002Encodes the spCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system
123902MTK234_008Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system
123903MTK234_010Encodes the spCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system
123904MTK234_016Encodes the saCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system
123905MTK234_021Encodes the spCas9 pUAS gRNA expression cassette as a type 234 part to be used in the MTK system
123906MTK234_026Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system
123907MTK234_027Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123908MTK234_028Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123909MTK234_029Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123910MTK234_030Encodes the spCas9 sgRNA1 hCLYBL (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123911MTK234_031Encodes the spCas9 sgRNA1 hCLYBL (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123912MTK234_037Encodes the spCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system
123913MTK234_043Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123914MTK234_039Encodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK system
123915MTK234_040Encodes the spCas9 mAzamiGreen gRNA expression cassette as a type 234 part to be used in the MTK system
123916MTK234_041Encodes the spCas9 mScarlet gRNA expression cassette as a type 234 part to be used in the MTK system
123917MTK234_038Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123918MTK234_050Encodes the saCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system
123919MTK234_051Encodes the saCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system
123920MTK234_055Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
123921MTK234_056Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system
123923MTK678_002Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system
123924MTK0_001Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
123925MTK0_003Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system
123927MTK0_007Encodes the AAV GFP dropout destination vector as a type 0 part to be used in the MTK system
123928MTK0_013Encodes the phiC31 attB GFP dropout destination vector as a type 0 part to be used in the MTK system
123929MTK0_014Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system
123930MTK0_015Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
123931MTK0_016Encodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
123932MTK0_017Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
123933MTK0_018Encodes the spacer TU with connectors LS R1 as a type 0 part to be used in the MTK system
123934MTK0_019Encodes the spacer TU with connectors L1 R2 as a type 0 part to be used in the MTK system
123935MTK0_020Encodes the spacer TU with connectors L2 R3 as a type 0 part to be used in the MTK system
123936MTK0_021Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system
123937MTK0_022Encodes the spacer TU with connectors L4 R5 as a type 0 part to be used in the MTK system
123938MTK0_023Encodes the spacer TU with connectors L5 R6 as a type 0 part to be used in the MTK system
123939MTK0_024Encodes the spacer TU with connectors L6 R7 as a type 0 part to be used in the MTK system
123940MTK0_025Encodes the spacer TU with connectors L7 R8 as a type 0 part to be used in the MTK system
123941MTK0_026Encodes the spacer TU with connectors L8 RE as a type 0 part to be used in the MTK system
123943MTK0_043Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system
123946MTK0_049Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system
123947MTK0_050Encodes the hCLYBL landing pad destination vector as a type 0 part to be used in the MTK system
123948MTK0_051Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123950MTK0_057Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123951MTK0_062Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

Antibodies from Article