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A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells.
Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123671MTK1_008Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123672MTK1_009Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123673MTK1_010Encodes ConL2 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123674MTK1_011Encodes ConL3 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123675MTK1_012Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123676MTK1_013Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123677MTK1_014Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system
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123678MTK1_015Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system
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123679MTK1_016Encodes ConL6 as a Type 1 part to be used in the MTK system
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123680MTK1_017Encodes ConL7 as a Type 1 part to be used in the MTK system
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123681MTK1_018Encodes ConL8 as a Type 1 part to be used in the MTK system
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123682MTK1_019Encodes ConL6 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123683MTK1_020Encodes ConL7 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system
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123684MTK1_022Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system
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123685MTK1_023Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system
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123686MTK1_024Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system
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123687MTK1_025Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system
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123688MTK1_026Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system
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123689MTK1_027Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system
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123690MTK1_028Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system
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123691MTK1_029Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system
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123692MTK1_030Encodes ConL1 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
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123693MTK1_031Encodes ConL2 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
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123694MTK1_032Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
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123695MTK1_033Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
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123696MTK1_034Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system
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123697MTK2_001Encodes pTRE as a Type 2 part to be used in the MTK system
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123698MTK2_003Encodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK system
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123699MTK2_004Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system
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123700MTK2_005Encodes pCAG as a Type 2 part to be used in the MTK system
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123701MTK2_006Encodes pSV40 as a Type 2 part to be used in the MTK system
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123702MTK2_007Encodes human pEF1alpha as a Type 2 part to be used in the MTK system
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123703MTK2_008Encodes human pUbc as a Type 2 part to be used in the MTK system
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123704MTK2_012Encodes a spacer as a Type 2 part to be used in the MTK system
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123705MTK2_013Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system
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123706MTK2_014Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system
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123707MTK2_015Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK sytem
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123708MTK2_016Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123709MTK2_017Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123710MTK2_018Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123711MTK2_019Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123712MTK2_020Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123713MTK2_021Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123714MTK2_022Encodes truncated pCMV as a Type 2 part to be used in the MTK system
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123715MTK2_023Encodes pCMV as a Type 2 part to be used in the MTK system
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123716MTK2_024Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system
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123717MTK2_025Encodes crippled human pEF1alpha as a Type 2 part to be used in the MTK system
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123718MTK2_026Encodes crippled pSFFV as a Type 2 part to be used in the MTK system
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123719MTK3_002Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system
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123720MTK3_004Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system
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123721MTK3_005Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system
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123722MTK3_006Encodes human histone H2B as a Type 3 part to be used in the MTK system
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123723MTK3_008Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system
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123724MTK3_009Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system
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123725MTK3_010Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system
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123726MTK3_011Encodes HA-tagged PIF6 fused to Tet Repressor protein as a Type 3 part to be used in the MTK system
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123727MTK3_012Encodes HA-tagged PYLcs fused to VP16 activator domain as a Type 3 part to be used in the MTK system
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123728MTK3_013Encodes Flag-tagged ABIcs fused to GAL4 DNA binding domain as a Type 3 part to be used in the MTK system
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123729MTK3_014Encodes Gq-coupled hM3D as a Type 3 part to be used in the MTK system
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123730MTK3_015Encodes Gi-coupled hM4D as a Type 3 part to be used in the MTK system
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123731MTK3_016Encodes Gs-coupled rM3D as a Type 3 part to be used in the MTK system
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123732MTK3_017Encodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK system
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123733MTK3_018Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system
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123734MTK3_019Encodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK system
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123735MTK3_020Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system
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123736MTK3_021Encodes ERK Translocation Reporter (ERK KTR) as a Type 3 part to be used in the MTK system
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123741MTK3_026Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system
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123742MTK3_027Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system
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123743MTK3_029Encodes PKA SPARK, a PKA activity reporter, as a Type 3 part to be used in the MTK system
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123744MTK3_030Encodes ERK SPARK, a ERK activity reporter, as a Type 3 part to be used in the MTK system
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123745MTK3_031Encodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as a Type 3 part to be used in the MTK system
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123746MTK3_032Encodes JNK Translocation Reporter (JNK KTR) fused to mRuby2 as a Type 3 part to be used in the MTK system
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123747MTK3_033Encodes Gibberellin Receptor fused to VPR activation domain as a Type 3 part to be used in the MTK system
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123748MTK3_034Encodes Absisic acid Receptor fused to VPR activation domain as a Type 3 part to be used in the MTK system
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123749MTK3_035Encodes human E-Cadherin as a Type 3 part to be used in the MTK system
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123750MTK3_036Encodes mScarlet as a Type 3 part to be used in the MTK system
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123751MTK3_037Encodes cAMPr, a cAMP reporter, as a Type 3 part to be used in the MTK system
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123752MTK3_038Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system
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123753MTK3_039Encodes mouse RelA as a Type 3 part to be used in the MTK system
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123754MTK3a_001Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK
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123755MTK3a_002Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system
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123756MTK3a_003Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system
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123757MTK3a_004Encodes Golgi Targeting Sequence as a Type 3a part to be used in the MTK system
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123758MTK3a_005Encodes Mitochondria Targeting Sequence as a Type 3a part to be used in the MTK system
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123759MTK3a_006Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be used in the MTK system
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123760MTK3a_007Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system
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123761MTK3a_008Encodes ERK Translocation Reporter (ERK KTR) as a Type 3a part to be used in the MTK system
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123762MTK3a_009Encodes BxBI attP site as a Type 3a part to be used in the MTK system
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123763MTK3a_010Encodes Phi31 attP site as a Type 3a part to be used in the MTK system
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123764MTK3a_011Encodes EGFP as a Type 3a part to be used in the MTK system
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123765MTK3a_013Encodes msGFP as a Type 3a part to be used in the MTK system
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123766MTK3a_014Encodes Kruppel Associated Box repression domain as a Type 3a part to be used in the MTK system
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123767MTK3a_015Encodes VPR activation domain as a Type 3a part to be used in the MTK system
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123768MTK3a_016Encodes Absisic acid insensitive 1 as a Type 3a part to be used in the MTK system
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123769MTK3a_017Encodes Gibberellin Insensitive as a Type 3a part to be used in the MTK system
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123770MTK3a_018Encodes human histone H2B as a Type 3a part to be used in the MTK system
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123771MTK3a_019Encodes Absisic acid receptor as a Type 3a part to be used in the MTK system
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123772MTK3a_020Encodes Gibberellin Receptor as a Type 3a part to be used in the MTK system
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123773MTK3a_021Encodes HELB-CDK2 Translocation Reporter as a Type 3a part to be used in the MTK system
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123774MTK3a_022Encodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK system
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123775MTK3a_023Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system
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123776MTK3a_024Encodes spCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
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123777MTK3a_025Encodes dspCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
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123778MTK3a_026Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system
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123779MTK3a_027Encodes tagBFP as a Type 3a part to be used in the MTK system
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123782MTK3b_001Encodes human beta actin as a Type 3b part to be used in the MTK system
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123783MTK3b_002Encodes human alpha tubulin 1 as a Type 3b part to be used in the MTK system
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123784MTK3b_003Encodes picornavirus peptide T2A element as a Type 3b part to be used in the MTK system
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123785MTK3b_004Encodes mAzamiGreen as a Type 3b part to be used in the MTK system
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123786MTK3b_005Encodes iRFP670 as a Type 3b part to be used in the MTK system
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123787MTK3b_006Encodes tagBFP as a Type 3b part to be used in the MTK system
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123788MTK3b_007Encodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 3b part to be used in the MTK system
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123789MTK3b_008Encodes EBFP2 as a Type 3b part to be used in the MTK system
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123791MTK3b_010Encodes mScarlet as a Type 3b part to be used in the MTK system
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123792MTK3b_011Encodes iRFP713 as a Type 3b part to be used in the MTK system
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123793MTK3b_012Encodes hph (hygromycin B phosphotransferase) as a Type 3b part to be used in the MTK system
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123794MTK3b_013Encodes Flamindo2,a cAMP reporter, as a Type 3 part to be used in the MTK system
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123795MTK3b_014Encodes picornavirus peptide P2A element as a Type 3b part to be used in the MTK system
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123796MTK3b_015Encodes rat Connexin 26 as a Type 3b part to be used in the MTK system
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123797MTK3b_016Encodes human Connexin 30 as a Type 3b part to be used in the MTK system
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123798MTK3b_017Encodes rat Connexin 43 as a Type 3b part to be used in the MTK system
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123799MTK3b_018Encodes dspCAS9 as a Type 3b part to be used in the MTK system
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123800MTK3b_019Encodes dsaCAS9 as a Type 3b part to be used in the MTK system
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123801MTK3b_020Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system
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123802MTK3b_021Encodes VPR activation domain as a Type 3b part to be used in the MTK system
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123803MTK3b_022Encodes Kruppel Associated Box repression domain as a Type 3b part to be used in the MTK system
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123804MTK3b_024Encodes human NFATC2 as a Type 3b part to be used in the MTK system
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123805MTK3b_027Encodes human TP53 as a Type 3b part to be used in the MTK system
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123806MTK3b_028Encodes Venus as a Type 3b part to be used in the MTK system
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123807MTK3b_029Encodes Tet-On 3G transactivator protein as a Type 3b part to be used in the MTK system
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123808MTK3b_030Encodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system
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123809MTK3b_031Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system
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123810MTK4_001Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system
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123811MTK4_002Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system
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123812MTK4_003Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system
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123813MTK4_004Encodes spacer sequence as a Type 4 part to be used in the MTK system
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123814MTK4_006Encodes spacer sequence (multicistronic) as a Type 4 part to be used in the MTK system
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123815MTK4a_001Encodes plasma membrane localization signal from Lyn kinase as a Type 4a part to be used in the MTK
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123816MTK4a_002Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system
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123817MTK4a_003Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system
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123819MTK4a_005Encodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK system
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123820MTK4a_006Encodes ER Targeting Sequence (N Terminal) as a Type 4a part to be used in the MTK system
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123821MTK4a_007Encodes Peroxisome Targeting Sequence as a Type 4a part to be used in the MTK system
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123822MTK4a_008Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system
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123823MTK4a_010Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system
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123824MTK4a_011Encodes a nuclear localized iRFP670 coexpression cassette as a Type 4a part to be used in the MTK system
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123825MTK4a_012Encodes mScarlet as a Type 4a part to be used in the MTK system
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123826MTK4a_013Encodes a nuclear localized iRFP713 coexpression cassette as a Type 4a part to be used in the MTK system
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123827MTK4a_014Encodes a nuclear localized mAzamiGreen coexpression cassette as a Type 4a part to be used in the MTK system
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123828MTK4a_015Encodes msfGFP as a Type 4a part to be used in the MTK system
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123829MTK4a_016Encodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK system
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123830MTK4a_017Encodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK system
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123831MTK4a_018Encodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK system
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123832MTK4a_019Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system
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123833MTK4a_020Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system
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123834MTK4a_021Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system
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123835MTK4a_023Encodes Gibberellin Insensitive as a Type 4a part to be used in the MTK system
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123836MTK4a_024Encodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system
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123838MTK4a_026Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system
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123839MTK4a_027Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system
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123840MTK4b_001Encodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK system
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123841MTK4b_002Encodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK system
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123842MTK4b_003Encodes rabbit beta-globin polyadenylation signal as a Type 4b part to be used in the MTK system
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123843MTK4b_004Encodes spacer sequence as a Type 4b part to be used in the MTK system
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123844MTK4b_006Encodes spacer sequence (multicistronic) as a Type 4b part to be used in the MTK system
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123852MTK5_008Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system
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123853MTK5_009Encodes ConR6 as a Type 5 part to be used in the MTK system
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123854MTK5_010Encodes ConR7 as a Type 5 part to be used in the MTK system
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123855MTK5_011Encodes ConR8 as a Type 5 part to be used in the MTK system
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123856MTK5_012Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system
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123857MTK6_003Encodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK system
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123858MTK6_004Encodes tagBFP, thosea asigna virus 2A (T2A) and Blasticidin resistance as a multicistronic cassette to be used in the MTK system
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123859MTK6_005Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette to be used in the MTK system
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123860MTK6_006Encodes a spacer cassette as a type 6 part to be used in the MTK system
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123861MTK6_007Encodes the attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK system
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123862MTK6_008Encodes the attB BxBI transposon recognition site cassette as a type 6 part to be used in the MTK system
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123863MTK6_009Encodes pCMV-Hygromycin resistance cassette as a type 6 part to be used in the MTK system
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123864MTK6_010Encodes FRT recognition site cassette as a type 6 part to be used in the MTK system
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123865MTK6_011Encodes the inverted attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK system
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123866MTK6_012Encodes the inverted attB BxBI transposon recognition site cassette as a type 6 part to be used in the MTK system
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123867MTK6_013Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system
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123868MTK6_014Encodes WPRE expressing tagBFP T2A Blasticidin resistance cassette without a terminator to be used in the MTK system
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123869MTK6_015Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system
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123870MTK7_001Encodes the 3' PiggyBac transposon recognition site cassette as a type 7 part to be used in the MTK system
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123871MTK7_002Encodes the 5' 500 bp hRosa26 locus homology arm as a type 7 part to be used in the MTK system
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123872MTK7_004Encodes the 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system
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123873MTK7_005Encodes the 3' AAV ITR cassette as a type 7 part to be used in the MTK system
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123874MTK7_006Encodes the FRT cassette as a type 7 part to be used in the MTK system
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123875MTK7_007Encodes the promoter-less Blasticidin cassette as a type 7 part to be used in the MTK system
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123876MTK7_009Encodes the 5' 500 bp cRosa26 locus homology arm as a type 7 part to be used in the MTK system
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123877MTK7_010Encodes promoter-less tagBFP T2A Blasticidin resistance cassette to be used in the MTK system
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123878MTK7_011Encodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK system
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123879MTK7_012Encodes the 5' 500 bp hCYBYL locus homology arm as a type 7 part to be used in the MTK system
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123880MTK7_013Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system
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123881MTK7_014Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system
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123884MTK8_004Encodes the F1 Ampicillin resistance cassette as a type 8 part to be used in the MTK system
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123885MTK8_005Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system
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123888MTK8a_004Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system
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123889MTK8a_005Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system
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123890MTK8b_001Encodes the 5' PiggyBac transposon recognition site cassette as a type 8b part to be used in the MTK system
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123891MTK8b_002Encodes the 3' 500 bp hRosa26 locus homology arm as a type 8b part to be used in the MTK system
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123892MTK8b_004Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system
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123893MTK8b_005Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system
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123894MTK8b_006Encodes the LoxP recognition site as a type 8b part to be used in the MTK system
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123895MTK8b_008Encodes the 3' 500 bp cRosa26 locus homology arm as a type 8b part to be used in the MTK system
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123896MTK8b_009Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system
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123897MTK8b_010Encodes the 3' 500 bp hCLYBL locus homology arm as a type 8b part to be used in the MTK system
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123898MTK8b_011Encodes the 3' 500 bp hAAVS1 locus homology arm as a type 8b part to be used in the MTK system
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123900MTK234_002Encodes the spCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system
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123902MTK234_008Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system
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123903MTK234_010Encodes the spCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system
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123904MTK234_016Encodes the saCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system
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123905MTK234_021Encodes the spCas9 pUAS gRNA expression cassette as a type 234 part to be used in the MTK system
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123906MTK234_026Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system
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123907MTK234_027Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
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123908MTK234_028Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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123909MTK234_029Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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123910MTK234_030Encodes the spCas9 sgRNA1 hCLYBL (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
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123911MTK234_031Encodes the spCas9 sgRNA1 hCLYBL (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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123912MTK234_037Encodes the spCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system
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123914MTK234_039Encodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK system
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123915MTK234_040Encodes the spCas9 mAzamiGreen gRNA expression cassette as a type 234 part to be used in the MTK system
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123916MTK234_041Encodes the spCas9 mScarlet gRNA expression cassette as a type 234 part to be used in the MTK system
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123918MTK234_050Encodes the saCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system
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123919MTK234_051Encodes the saCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system
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123920MTK234_055Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
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123921MTK234_056Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system
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123923MTK678_002Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system
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123924MTK0_001Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
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123925MTK0_003Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system
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123927MTK0_007Encodes the AAV GFP dropout destination vector as a type 0 part to be used in the MTK system
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123928MTK0_013Encodes the phiC31 attB GFP dropout destination vector as a type 0 part to be used in the MTK system
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123929MTK0_014Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system
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123930MTK0_015Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
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123931MTK0_016Encodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
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123932MTK0_017Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
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123933MTK0_018Encodes the spacer TU with connectors LS R1 as a type 0 part to be used in the MTK system
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123934MTK0_019Encodes the spacer TU with connectors L1 R2 as a type 0 part to be used in the MTK system
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123935MTK0_020Encodes the spacer TU with connectors L2 R3 as a type 0 part to be used in the MTK system
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123936MTK0_021Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system
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123937MTK0_022Encodes the spacer TU with connectors L4 R5 as a type 0 part to be used in the MTK system
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123938MTK0_023Encodes the spacer TU with connectors L5 R6 as a type 0 part to be used in the MTK system
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123939MTK0_024Encodes the spacer TU with connectors L6 R7 as a type 0 part to be used in the MTK system
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123940MTK0_025Encodes the spacer TU with connectors L7 R8 as a type 0 part to be used in the MTK system
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123941MTK0_026Encodes the spacer TU with connectors L8 RE as a type 0 part to be used in the MTK system
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123943MTK0_043Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system
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123946MTK0_049Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system
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123947MTK0_050Encodes the hCLYBL landing pad destination vector as a type 0 part to be used in the MTK system
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123948MTK0_051Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
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123950MTK0_057Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
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123951MTK0_062Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system
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123952MTK3_001Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system
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123953MTK3b_023Encodes human RelA as a Type 3b part to be used in the MTK system
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123954MTK3b_025Encodes synTF fused to VP16 activation domain as a Type 3b part to be used in the MTK system
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123955MTK3b_026Encodes synTF fused to VP64 activation domain as a Type 3b part to be used in the MTK system
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123956MTK4a_009Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system
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123957MTK234_009Encodes the spCas9 pC120 gRNA expression cassette as a type 234 part to be used in the MTK system
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123958MTK234_011Encodes the spCas9 pPLMP1 gRNA expression cassette as a type 234 part to be used in the MTK system
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123959MTK234_054Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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123960MTK0_002Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system
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123961MTK0_004Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
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123964MTK0_009Encodes PhiC31 landing pad as a type 0 part to be used in the MTK system
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123965MTK0_010Encodes BxBI landing pad as a type 0 part to be used in the MTK system
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123966MTK0_011Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
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123967MTK0_012Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
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123968MTK0_028Encodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
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123969MTK0_030Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
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123970MTK0_031Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
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123971MTK0_033Encodes the cROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
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123972MTK0_034Encodes the hROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
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123973MTK0_036Encodes the cROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
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123974MTK0_037Encodes the hROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
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123975MTK0_045Encodes the cROSA26 destination with hygyomycin resistance cassette as a type 0 part to be used in the MTK system
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123976MTK0_046Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
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123977MTK0_047Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
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123978MTK0_052Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
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123980MTK0_054Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
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123982MTK234_057Encodes the spCas9 Safetargeting Chr20 gRNA expression cassette as a type 234 part to be used in the MTK system
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123988MTK234_012Encodes the spCas9 sgRNA1 hROSA26 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
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123989MTK234_013Encodes the spCas9 sgRNA1 hROSA26 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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123990MTK234_014Encodes the spCas9 sgRNA1 hROSA26 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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123991MTK234_015Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
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123992MTK234_005Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system
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123993MTK234_006Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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123994MTK234_007Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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123995MTK234_032Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
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123996MTK234_033Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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123997MTK234_034Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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123998JPF0507Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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123999JPF0508Encodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124000JPF0509Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124001JPF0510Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124002JPF0511Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124003JPF0512Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124004JPF0513Encodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124005JPF0514Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124006JPF0515Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124007JPF0516Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124008JPF0517Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124009JPF0518Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124010JPF0519Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124011JPF0520Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124012JPF0521Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124013JPF0522Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124014JPF0523Encodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
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124015ARB287aEncodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
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124016ARB287bEncodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
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124017ARB287cEncodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
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124018ARB287dEncodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
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124019ARB287eEncodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
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124020JPF0419dEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
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124021JPF0419vEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
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124022JPF0446BxBI Destination vector with H2B::GFP expressed from CAG promoter
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124023ARB340Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713
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124024ARB341Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP
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124025ARB342Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen
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124026ARB343Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2
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124027ARB344Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713
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124028ARB345Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP
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124029ARB346Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen
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124030ARB347Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2
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124031ARB348Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged iRFP713
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124032ARB349Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFP
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124033ARB350Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mAzamiGreen
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124034ARB351Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2
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124035JPF0464Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
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124036JPF0465Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
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124037JPF0466Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
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124038JPF0468Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
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124039JPF0454Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector
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124040JPF0455Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector
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124041JPF0456Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector
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124042JPF0535aEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
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124048ARB365Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
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124049ARB366Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
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124050ARB367Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
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124054MTK3_028Encodes c120 sg RNA guide cleaved by ribozyme
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124055MTK234_047Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
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124056MTK234_048Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
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124057MTK234_049Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
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124058MTK6_016Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system
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