Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

plasmid kit icon

The Mammalian Toolkit
(Kit # 1000000180 )

Depositing Lab:   Hana El-Samad

This toolkit is a ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$900 USD + shipping

Available to academics and nonprofits only.

Original Publication

Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. PubMed: PMID: 31686495 .

Description

This kit includes 253 plasmids from the Mammalian Toolkit. Additional plasmids described in this publication, but not included in the kit, can be requested as individual bacterial stabs. These plasmids allow for the assembly of cellular circuits using Golden Gate-based cloning methods for use in mammalian cells. Please see both the associated publication and protocol publication for additional information on the use of these plasmids.

  • Construction of circuits using plasmids from the Mammalian Toolkit (MTK) for use in viral, integration, and homologous recombination experiments in mammalian cells.

Protocol

Assembly of Genetic Circuits with the Mammalian ToolKit. Fonseca JP, Bonny AR, Town J, El-Samad H. Bio Protoc. 2020 Mar 5;10(5):e3547. doi: 10.21769/BioProtoc.3547. PubMed: PMID: 33659521 .

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Mammalian Toolkit was a gift from Hana El-Samad (Addgene kit #1000000180).”

For your Reference section:

Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. PubMed: PMID: 31686495 .

Assembly of Genetic Circuits with the Mammalian ToolKit. Fonseca JP, Bonny AR, Town J, El-Samad H. Bio Protoc. 2020 Mar 5;10(5):e3547. doi: 10.21769/BioProtoc.3547. PubMed: PMID: 33659521 .

The Mammalian Toolkit - #1000000180

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol
Kanamycin
Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 MTK1_008
Chloramphenicol
Plate 1 / A / 2 MTK1_009
Chloramphenicol
Plate 1 / A / 3 MTK1_010
Chloramphenicol
Plate 1 / A / 4 MTK1_011
Chloramphenicol
Plate 1 / A / 5 MTK1_012
Chloramphenicol
Plate 1 / A / 6 MTK1_013
Chloramphenicol
Plate 1 / A / 7 MTK1_014
Chloramphenicol
Plate 1 / A / 8 MTK1_015
Chloramphenicol
Plate 1 / A / 9 MTK1_016
Chloramphenicol
Plate 1 / A / 10 MTK1_017
Chloramphenicol
Plate 1 / A / 11 MTK1_018
Chloramphenicol
Plate 1 / A / 12 MTK1_019
Chloramphenicol
Plate 1 / B / 1 MTK1_020
Chloramphenicol
Plate 1 / B / 2 MTK1_022
Chloramphenicol
Plate 1 / B / 3 MTK1_023
Chloramphenicol
Plate 1 / B / 4 MTK1_024
Chloramphenicol
Plate 1 / B / 5 MTK1_025
Chloramphenicol
Plate 1 / B / 6 MTK1_026
Chloramphenicol
Plate 1 / B / 7 MTK1_027
Chloramphenicol
Plate 1 / B / 8 MTK1_028
Chloramphenicol
Plate 1 / B / 9 MTK1_029
Chloramphenicol
Plate 1 / B / 10 MTK1_030
Chloramphenicol
Plate 1 / B / 11 MTK1_031
Chloramphenicol
Plate 1 / B / 12 MTK1_032
Chloramphenicol
Plate 1 / C / 1 MTK1_033
Chloramphenicol
Plate 1 / C / 2 MTK1_034
Chloramphenicol
Plate 1 / C / 3 MTK2_001
Chloramphenicol
Plate 1 / C / 4 MTK2_003
Chloramphenicol
Plate 1 / C / 5 MTK2_004
Chloramphenicol
Plate 1 / C / 6 MTK2_005
Chloramphenicol
Plate 1 / C / 7 MTK2_006
Chloramphenicol
Plate 1 / C / 8 MTK2_007
Chloramphenicol
Plate 1 / C / 9 MTK2_008
Chloramphenicol
Plate 1 / C / 10 MTK2_012
Chloramphenicol
Plate 1 / C / 11 MTK2_013
Chloramphenicol
Plate 1 / C / 12 MTK2_014
Chloramphenicol
Plate 1 / D / 1 MTK2_015
Chloramphenicol
Plate 1 / D / 2 MTK2_016
Chloramphenicol
Plate 1 / D / 3 MTK2_017
Chloramphenicol
Plate 1 / D / 4 MTK2_018
Chloramphenicol
Plate 1 / D / 5 MTK2_019
Chloramphenicol
Plate 1 / D / 6 MTK2_020
Chloramphenicol
Plate 1 / D / 7 MTK2_021
Chloramphenicol
Plate 1 / D / 8 MTK2_022
Chloramphenicol
Plate 1 / D / 9 MTK2_023
Chloramphenicol
Plate 1 / D / 10 MTK2_024
Chloramphenicol
Plate 1 / D / 11 MTK2_025
Chloramphenicol
Plate 1 / D / 12 MTK2_026
Chloramphenicol
Plate 1 / E / 1 MTK3_002
Chloramphenicol
Plate 1 / E / 2 MTK3_004
Chloramphenicol
Plate 1 / E / 3 MTK3_005
Chloramphenicol
Plate 1 / E / 4 MTK3_006
Chloramphenicol
Plate 1 / E / 5 MTK3_008
Chloramphenicol
Plate 1 / E / 6 MTK3_009
Chloramphenicol
Plate 1 / E / 7 MTK3_010
Chloramphenicol
Plate 1 / E / 8 MTK3_011
Chloramphenicol
Plate 1 / E / 9 MTK3_012
Chloramphenicol
Plate 1 / E / 10 MTK3_013
Chloramphenicol
Plate 1 / E / 11 MTK3_014
Chloramphenicol
Plate 1 / E / 12 MTK3_015
Chloramphenicol
Plate 1 / F / 1 MTK3_016
Chloramphenicol
Plate 1 / F / 2 MTK3_017
Chloramphenicol
Plate 1 / F / 3 MTK3_018
Chloramphenicol
Plate 1 / F / 4 MTK3_019
Chloramphenicol
Plate 1 / F / 5 MTK3_020
Chloramphenicol
Plate 1 / F / 6 MTK3_021
Chloramphenicol
Plate 1 / F / 7 MTK3_026
Chloramphenicol
Plate 1 / F / 8 MTK3_027
Chloramphenicol
Plate 1 / F / 9 MTK3_029
Chloramphenicol
Plate 1 / F / 10 MTK3_030
Chloramphenicol
Plate 1 / F / 11 MTK3_031
Chloramphenicol
Plate 1 / F / 12 MTK3_032
Chloramphenicol
Plate 1 / G / 1 MTK3_033
Chloramphenicol
Plate 1 / G / 2 MTK3_034
Chloramphenicol
Plate 1 / G / 3 MTK3_035
Chloramphenicol
Plate 1 / G / 4 MTK3_036
Chloramphenicol
Plate 1 / G / 5 MTK3_037
Chloramphenicol
Plate 1 / G / 6 MTK3_038
Chloramphenicol
Plate 1 / G / 7 MTK3_039
Chloramphenicol
Plate 1 / G / 8 MTK3a_001
Chloramphenicol
Plate 1 / G / 9 MTK3a_002
Chloramphenicol
Plate 1 / G / 10 MTK3a_003
Chloramphenicol
Plate 1 / G / 11 MTK3a_004
Chloramphenicol
Plate 1 / G / 12 MTK3a_005
Chloramphenicol
Plate 1 / H / 1 MTK3a_006
Chloramphenicol
Plate 1 / H / 2 MTK3a_007
Chloramphenicol
Plate 1 / H / 3 MTK3a_008
Chloramphenicol
Plate 1 / H / 4 MTK3a_009
Chloramphenicol
Plate 1 / H / 5 MTK3a_010
Chloramphenicol
Plate 1 / H / 6 MTK3a_011
Chloramphenicol
Plate 1 / H / 7 MTK3a_013
Chloramphenicol
Plate 1 / H / 8 MTK3a_014
Chloramphenicol
Plate 1 / H / 9 MTK3a_015
Chloramphenicol
Plate 1 / H / 10 MTK3a_016
Chloramphenicol
Plate 1 / H / 11 MTK3a_017
Chloramphenicol
Plate 1 / H / 12 MTK3a_018
Chloramphenicol
Plate 2 / A / 1 MTK3a_019
Chloramphenicol
Plate 2 / A / 2 MTK3a_020
Chloramphenicol
Plate 2 / A / 3 MTK3a_021
Chloramphenicol
Plate 2 / A / 4 MTK3a_022
Chloramphenicol
Plate 2 / A / 5 MTK3a_023
Chloramphenicol
Plate 2 / A / 6 MTK3a_024
Chloramphenicol
Plate 2 / A / 7 MTK3a_025
Chloramphenicol
Plate 2 / A / 8 MTK3a_026
Chloramphenicol
Plate 2 / A / 9 MTK3a_027
Chloramphenicol
Plate 2 / A / 10 MTK3b_001
Chloramphenicol
Plate 2 / A / 11 MTK3b_002
Chloramphenicol
Plate 2 / A / 12 MTK3b_003
Chloramphenicol
Plate 2 / B / 1 MTK3b_004
Chloramphenicol
Plate 2 / B / 2 MTK3b_005
Chloramphenicol
Plate 2 / B / 3 MTK3b_006
Chloramphenicol
Plate 2 / B / 4 MTK3b_007
Chloramphenicol
Plate 2 / B / 5 MTK3b_008
Chloramphenicol
Plate 2 / B / 6 MTK3b_010
Chloramphenicol
Plate 2 / B / 7 MTK3b_011
Chloramphenicol
Plate 2 / B / 8 MTK3b_012
Chloramphenicol
Plate 2 / B / 9 MTK3b_013
Chloramphenicol
Plate 2 / B / 10 MTK3b_014
Chloramphenicol
Plate 2 / B / 11 MTK3b_015
Chloramphenicol
Plate 2 / B / 12 MTK3b_016
Chloramphenicol
Plate 2 / C / 1 MTK3b_017
Chloramphenicol
Plate 2 / C / 2 MTK3b_018
Chloramphenicol
Plate 2 / C / 3 MTK3b_019
Chloramphenicol
Plate 2 / C / 4 MTK3b_020
Chloramphenicol
Plate 2 / C / 5 MTK3b_021
Chloramphenicol
Plate 2 / C / 6 MTK3b_022
Chloramphenicol
Plate 2 / C / 7 MTK3b_024
Chloramphenicol
Plate 2 / C / 8 MTK3b_027
Chloramphenicol
Plate 2 / C / 9 MTK3b_028
Chloramphenicol
Plate 2 / C / 10 MTK3b_029
Chloramphenicol
Plate 2 / C / 11 MTK3b_030
Chloramphenicol
Plate 2 / C / 12 MTK3b_031
Chloramphenicol
Plate 2 / D / 1 MTK4_001
Chloramphenicol
Plate 2 / D / 2 MTK4_002
Chloramphenicol
Plate 2 / D / 3 MTK4_003
Chloramphenicol
Plate 2 / D / 4 MTK4_004
Chloramphenicol
Plate 2 / D / 5 MTK4_006
Chloramphenicol
Plate 2 / D / 6 MTK4a_001
Chloramphenicol
Plate 2 / D / 7 MTK4a_002
Chloramphenicol
Plate 2 / D / 8 MTK4a_003
Chloramphenicol
Plate 2 / D / 9 MTK4a_005
Chloramphenicol
Plate 2 / D / 10 MTK4a_006
Chloramphenicol
Plate 2 / D / 11 MTK4a_007
Chloramphenicol
Plate 2 / D / 12 MTK4a_008
Chloramphenicol
Plate 2 / E / 1 MTK4a_010
Chloramphenicol
Plate 2 / E / 2 MTK4a_011
Chloramphenicol
Plate 2 / E / 3 MTK4a_012
Chloramphenicol
Plate 2 / E / 4 MTK4a_013
Chloramphenicol
Plate 2 / E / 5 MTK4a_014
Chloramphenicol
Plate 2 / E / 6 MTK4a_015
Chloramphenicol
Plate 2 / E / 7 MTK4a_016
Chloramphenicol
Plate 2 / E / 8 MTK4a_017
Chloramphenicol
Plate 2 / E / 9 MTK4a_018
Chloramphenicol
Plate 2 / E / 10 MTK4a_019
Chloramphenicol
Plate 2 / E / 11 MTK4a_020
Chloramphenicol
Plate 2 / E / 12 MTK4a_021
Chloramphenicol
Plate 2 / F / 1 MTK4a_023
Chloramphenicol
Plate 2 / F / 2 MTK4a_024
Chloramphenicol
Plate 2 / F / 3 MTK4a_026
Chloramphenicol
Plate 2 / F / 4 MTK4a_027
Chloramphenicol
Plate 2 / F / 5 MTK4b_001
Chloramphenicol
Plate 2 / F / 6 MTK4b_002
Chloramphenicol
Plate 2 / F / 7 MTK4b_003
Chloramphenicol
Plate 2 / F / 8 MTK4b_004
Chloramphenicol
Plate 2 / F / 9 MTK4b_006
Chloramphenicol
Plate 2 / F / 10 MTK5_008
Chloramphenicol
Plate 2 / F / 11 MTK5_009
Chloramphenicol
Plate 2 / F / 12 MTK5_010
Chloramphenicol
Plate 2 / G / 1 MTK5_011
Chloramphenicol
Plate 2 / G / 2 MTK5_012
Chloramphenicol
Plate 2 / G / 3 MTK6_003
Chloramphenicol
Plate 2 / G / 4 MTK6_004
Chloramphenicol
Plate 2 / G / 5 MTK6_005
Chloramphenicol
Plate 2 / G / 6 MTK6_006
Chloramphenicol
Plate 2 / G / 7 MTK6_007
Chloramphenicol
Plate 2 / G / 8 MTK6_008
Chloramphenicol
Plate 2 / G / 9 MTK6_009
Chloramphenicol
Plate 2 / G / 10 MTK6_010
Chloramphenicol
Plate 2 / G / 11 MTK6_011
Chloramphenicol
Plate 2 / G / 12 MTK6_012
Chloramphenicol
Plate 2 / H / 1 MTK6_013
Chloramphenicol
Plate 2 / H / 2 MTK6_014
Chloramphenicol
Plate 2 / H / 3 MTK6_015
Chloramphenicol
Plate 2 / H / 4 MTK7_001
Chloramphenicol
Plate 2 / H / 5 MTK7_002
Chloramphenicol
Plate 2 / H / 6 MTK7_004
Chloramphenicol
Plate 2 / H / 7 MTK7_005
Chloramphenicol
Plate 2 / H / 8 MTK7_006
Chloramphenicol
Plate 2 / H / 9 MTK7_007
Chloramphenicol
Plate 2 / H / 10 MTK7_009
Chloramphenicol
Plate 2 / H / 11 MTK7_010
Chloramphenicol
Plate 2 / H / 12 MTK7_011
Chloramphenicol
Plate 3 / A / 1 MTK7_012
Chloramphenicol
Plate 3 / A / 2 MTK7_013
Chloramphenicol
Plate 3 / A / 3 MTK7_014
Chloramphenicol
Plate 3 / A / 4 MTK8b_001
Chloramphenicol
Plate 3 / A / 5 MTK8b_002
Chloramphenicol
Plate 3 / A / 6 MTK8b_004
Chloramphenicol
Plate 3 / A / 7 MTK8b_005
Chloramphenicol
Plate 3 / A / 8 MTK8b_006
Chloramphenicol
Plate 3 / A / 9 MTK8b_008
Chloramphenicol
Plate 3 / A / 10 MTK8b_009
Chloramphenicol
Plate 3 / A / 11 MTK8b_010
Chloramphenicol
Plate 3 / A / 12 MTK8b_011
Chloramphenicol
Plate 3 / B / 1 MTK234_002
Chloramphenicol
Plate 3 / B / 2 MTK234_008
Chloramphenicol
Plate 3 / B / 3 MTK234_010
Chloramphenicol
Plate 3 / B / 4 MTK234_016
Chloramphenicol
Plate 3 / B / 5 MTK234_021
Chloramphenicol
Plate 3 / B / 6 MTK234_026
Chloramphenicol
Plate 3 / B / 7 MTK234_027
Chloramphenicol
Plate 3 / B / 8 MTK234_028
Chloramphenicol
Plate 3 / B / 9 MTK234_029
Chloramphenicol
Plate 3 / B / 10 MTK234_030
Chloramphenicol
Plate 3 / B / 11 MTK234_031
Chloramphenicol
Plate 3 / B / 12 MTK234_037
Chloramphenicol
Plate 3 / C / 1 MTK234_043
Chloramphenicol
Plate 3 / C / 2 MTK234_039
Chloramphenicol
Plate 3 / C / 3 MTK234_040
Chloramphenicol
Plate 3 / C / 4 MTK234_041
Chloramphenicol
Plate 3 / C / 5 MTK234_038
Chloramphenicol
Plate 3 / C / 6 MTK234_050
Chloramphenicol
Plate 3 / C / 7 MTK234_051
Chloramphenicol
Plate 3 / C / 8 MTK234_055
Chloramphenicol
Plate 3 / C / 9 MTK234_056
Chloramphenicol
Plate 3 / C / 10 MTK8_005
Kanamycin
Plate 3 / C / 11 MTK8a_005
Kanamycin
Plate 3 / C / 12 MTK0_003
Kanamycin
Plate 3 / D / 1 MTK0_007
Kanamycin
Plate 3 / D / 2 MTK0_013
Kanamycin
Plate 3 / D / 3 MTK0_014
Kanamycin
Plate 3 / D / 4 MTK0_015
Kanamycin
Plate 3 / D / 5 MTK0_016
Kanamycin
Plate 3 / D / 6 MTK0_017
Kanamycin
Plate 3 / D / 7 MTK0_043
Kanamycin
Plate 3 / D / 8 MTK0_049
Kanamycin
Plate 3 / D / 9 MTK0_050
Kanamycin
Plate 3 / D / 10 MTK0_051
Kanamycin
Plate 3 / D / 11 MTK0_057
Kanamycin
Plate 3 / D / 12 MTK0_062
Kanamycin
Plate 3 / E / 1 MTK0_018
Ampicillin
Plate 3 / E / 2 MTK0_019
Ampicillin
Plate 3 / E / 3 MTK0_020
Ampicillin
Plate 3 / E / 4 MTK0_021
Ampicillin
Plate 3 / E / 5 MTK0_022
Ampicillin
Plate 3 / E / 6 MTK0_023
Ampicillin
Plate 3 / E / 7 MTK0_024
Ampicillin
Plate 3 / E / 8 MTK0_025
Ampicillin
Plate 3 / E / 9 MTK8_004
Ampicillin
Plate 3 / E / 10 MTK8a_004
Ampicillin
Plate 3 / E / 11 MTK0_026
Ampicillin
Plate 3 / E / 12 MTK678_002
Ampicillin
Plate 3 / F / 1 MTK0_001
Ampicillin
Data calculated @ 2022-05-20

Kit Plate Map - #1000000180

Content blocked, you may need to disable your ad-blocker.