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EMMA: Extensible Mammalian Modular Assembly Toolkit
(Kit # 1000000119 )

Depositing Lab:   Yizhi Cai

The EMMA (extensible mammalian modular assembly) Toolkit contains a set of assembly connectors, a receiver vector, and a set of standardized genetic parts that can be used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction. EMMA is compatible with automated production and provides new opportunities for mammalian synthetic biology.

This kit will be shipped as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$400 USD + shipping

Available to academics and nonprofits only.

Original Publication

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors Martella A, Matjusaitis M, Auxillos J, Pollard SM, and Cai Y. ACS Synth Biol. 2017 Jul 21;6(7):1380-1392. doi: 10.1021/acssynbio.7b00016. PMID: 28418644 .

Description

The EMMA Toolkit contains a set of assembly connectors, a receiver vector, and a set of standardized genetic parts that can be used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction. Vectors for transient and stable transfections can be designed joining the functional modules of the EMMA platform using procedures described in the paper. The toolkit also provides a set of part-entry vectors that can be used to easily standardize new genetic elements and therefore expand the library of genetic parts.

EMMA is compatible with automated production and provides new opportunities for mammalian synthetic biology.

EMMA_toolkit.png
Schematic representation of EMMA toolkit- workflow for design and construction of mammalian expression vectors

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The EMMA toolkit was a gift from Yizhi Cai (Addgene kit # 1000000119).”

For your Reference section:

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors Martella A. et al. ACS Synth Biol. 2017 Jul 21;6(7):1380-1392. doi: 10.1021/acssynbio.7b00016. PMID: 28418644 .

EMMA toolkit - #1000000119

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Kanamycin
Ampicillin

Inventory

Well Plasmid Resistance
A / 1 YCe1693 HC_Kan_RFP-p1
Kanamycin
A / 2 YCe1500 HC_Kan_RFP-p2
Kanamycin
A / 3 YCe1502 HC_Kan_RFP-p3
Kanamycin
A / 4 YCe1505 HC_Kan_RFP-p4
Kanamycin
A / 5 YCe1506 HC_Kan_RFP-p5
Kanamycin
A / 6 YCe1509 HC_Kan_RFP-p6
Kanamycin
A / 7 YCe1695 HC_Kan_RFP-p7
Kanamycin
A / 8 YCe1697 HC_Kan_RFP-p8
Kanamycin
A / 9 YCe1730 HC_Kan_RFP-p8a
Kanamycin
A / 10 YCe1717 HC_Kan_RFP-p8b
Kanamycin
A / 11 YCe1699 HC_Kan_RFP-p9
Kanamycin
A / 12 YCe1701 HC_Kan_RFP-p10
Kanamycin
B / 1 YCe1703 HC_Kan_RFP-p11
Kanamycin
B / 2 YCe1705 HC_Kan_RFP-p12
Kanamycin
B / 3 YCe1707 HC_Kan_RFP-p13
Kanamycin
B / 4 YCe1709 HC_Kan_RFP-p14
Kanamycin
B / 5 YCe1719 HC_Kan_RFP-p15
Kanamycin
B / 6 YCe1486 HC_Kan_RFP-p16
Kanamycin
B / 7 YCe1487 HC_Kan_RFP-p17
Kanamycin
B / 8 YCe1488 HC_Kan_RFP-p18
Kanamycin
B / 9 YCe1491 HC_Kan_RFP-p19
Kanamycin
B / 10 YCe1711 HC_Kan_RFP-p20
Kanamycin
B / 11 YCe1713 HC_Kan_RFP-p21
Kanamycin
B / 12 YCe1514 HC_Kan_RFP-p22
Kanamycin
C / 1 YCe1715 HC_Kan_RFP-p23
Kanamycin
C / 2 YCe1731 HC_Kan_RFP-p24
Kanamycin
C / 3 YCe1721 HC_Kan_RFP-p25
Kanamycin
C / 4 YCe2764 HC_Kan_RFP-Connector entry vector
Kanamycin
C / 5 YCe3736_HC_Amp_ccdB_receiver vector
Ampicillin
C / 6 YCe3731 HC_Kan_conn_A-B
Kanamycin
C / 7 YCe3732 HC_Kan_conn_B-C
Kanamycin
C / 8 YCe1915 HC_Kan_conn_A-C
Kanamycin
C / 9 YCe2024 HC_Kan_conn_D-E
Kanamycin
C / 10 YCe3756 HC_Kan_conn_E-F
Kanamycin
C / 11 YCe1917 HC_Kan_conn_D-F
Kanamycin
C / 12 YCe2982 HC_Kan_conn_B-E
Kanamycin
D / 1 YCe2984 HC_Kan_conn_B-L
Kanamycin
D / 2 YCe1921 HC_Kan_conn_H-K
Kanamycin
D / 3 YCe2986 HC_Kan_conn_H-J
Kanamycin
D / 4 YCe3734 HC_Kan_conn_Ha-J
Kanamycin
D / 5 YCe3735 HC_Kan_conn_Ha-K
Kanamycin
D / 6 YCe1913 HC_Kan_conn_H-Ha
Kanamycin
D / 7 YCe2022 HC_Kan_conn_J-K
Kanamycin
D / 8 YCe3760 HC_Kan_conn_L-M
Kanamycin
D / 9 YCe2718 HC_Kan_conn_L-N
Kanamycin
D / 10 YCe1920 HC_Kan_conn_L-R
Kanamycin
D / 11 YCe2731 HC_Kan_conn_L-W
Kanamycin
D / 12 YCe1918 HC_Kan_conn_L-Y
Kanamycin
E / 1 YCe3733 HC_Kan_conn_M-R
Kanamycin
E / 2 YCe3761 HC_Kan_conn_N-R
Kanamycin
E / 3 YCe2020 HC_Kan_conn_Q-R
Kanamycin
E / 4 YCe3762 HC_Kan_conn_Q-W
Kanamycin
E / 5 YCe2780 HC_Kan_conn_R-W
Kanamycin
E / 6 YCe2786 HC_Kan_conn_T-V
Kanamycin
E / 7 YCe3757 HC_Kan_conn_U-V
Kanamycin
E / 8 YCe2721 HC_Kan_conn_W-Z
Kanamycin
E / 9 YCe2733 HC_Kan_conn_W-Y
Kanamycin
E / 10 YCe3159 HC_Kan_conn_W-X
Kanamycin
E / 11 YCe2066 HC_Kan_conn_Y-Z
Kanamycin
E / 12 YCe4520 HC_Kan_conn_BsaI-1_p1
Kanamycin
F / 1 YCe2725 HC_Kan_conn_BsaI-2_p24
Kanamycin
F / 2 YCe4522 HC_Kan_conn_BsaI-3_p1
Kanamycin
F / 3 YCe2730 HC_Kan_conn_BsaI-4_p25
Kanamycin
F / 4 YCe1759 HC_Kan_CAGp_p3
Kanamycin
F / 5 YCe1760 HC_Kan_IRES2_p8b
Kanamycin
F / 6 YCe1762 HC_Kan_SV40p_p14
Kanamycin
F / 7 YCe1883 HC_Kan_p2A_p8
Kanamycin
F / 8 YCe1885 HC_Kan_Kozak-ATG-MLS_p6
Kanamycin
F / 9 YCe1889 HC_Kan_SV40-polyA_p11
Kanamycin
F / 10 YCe1891 HC_Kan_SV40-ORI_p25
Kanamycin
F / 11 YCe1895 HC_Kan_Kozak-ATG_p6
Kanamycin
F / 12 YCe1897 HC_Kan_C-KDEL_8a
Kanamycin
G / 1 YCe1899 HC_Kan_PuroR_p9
Kanamycin
G / 2 YCe1901 HC_Kan_PGKpA_p22
Kanamycin
G / 3 YCe1903 HC_Kan_SV40-NLS_p6
Kanamycin
G / 4 YCe1905 HC_Kan_DmrA_p21
Kanamycin
G / 5 YCe1911 HC_Kan_Insulator_FB_p2
Kanamycin
G / 6 YCe2026 HC_Kan_mRuby2_p9
Kanamycin
G / 7 YCe2030 HC_Kan_bGHpA_p16
Kanamycin
G / 8 YCe2034 HC_Kan_mTagBFP2_p9
Kanamycin
G / 9 YCe2038 HC_Kan_CMVp_p18
Kanamycin
G / 10 YCe2040 HC_Kan_PuroR_p21
Kanamycin
G / 11 YCe2046 HC_Kan_PuroR_p15
Kanamycin
G / 12 YCe2052 HC_Kan_DmrC_p9
Kanamycin
H / 1 YCe2058 HC_Kan_Kozak_ATG_Palm_p6
Kanamycin
H / 2 YCe2060 HC_Kan_Linker3_p20
Kanamycin
H / 3 YCe2064 HC_Kan_p2A_p20
Kanamycin
H / 4 YCe2735 HC_Kan_Kozak-ATG-IgkL_p6
Kanamycin
H / 5 YCe2741 HC_Kan_EF1ap_p18
Kanamycin
H / 6 YCe2746 HC_Kan_Linker1_p8
Kanamycin
H / 7 YCe2748 HC_Kan_5'ITR-PB_p1
Kanamycin
H / 8 YCe2750 HC_Kan_3'ITR-PB_p24
Kanamycin
H / 9 YCe3752 HC_Kan_IRES2_p20
Kanamycin
H / 10 YCe3766 HC_Kan_3'-LTR_p10
Kanamycin
H / 11 YCe3777 HC_Kan_Chimeric-5'LTR_p1
Kanamycin
Data calculated @ 2018-04-24

Kit Plate Map - #1000000119

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