Purposepart designed to occupy position 22 of EMMA. Functional category: Insulator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100686||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
FII/BEAD-A (FB) element. (STEMCELLS2008;26:3257�3266 ). FII/BEAD-A (FB) contains the minimal enhancer-blocking components of the chicken b-globin 5'HS4 insulator and a homologous region from the human T-cell receptor BEAD-1 insulator
IMPORT NOTES: Special character found in field(Comments)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe1911 HC_Kan_Insulator_FB_p2 was a gift from Yizhi Cai (Addgene plasmid # 100686 ; http://n2t.net/addgene:100686 ; RRID:Addgene_100686)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644