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EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.
Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
100609YCe1693 HC_Kan_RFP-p1EMMA toolkit part-entry vector position 1
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100610YCe1500 HC_Kan_RFP-p2EMMA toolkit part-entry vector position 2
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100611YCe1502 HC_Kan_RFP-p3EMMA toolkit part-entry vector position 3
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100612YCe1505 HC_Kan_RFP-p4EMMA toolkit part-entry vector position 4
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100613YCe1506 HC_Kan_RFP-p5EMMA toolkit part-entry vector position 5
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100614YCe1509 HC_Kan_RFP-p6EMMA toolkit part-entry vector position 6
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100615YCe1695 HC_Kan_RFP-p7EMMA toolkit part-entry vector position 7
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100616YCe1697 HC_Kan_RFP-p8EMMA toolkit part-entry vector position 8
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100617YCe1730 HC_Kan_RFP-p8aEMMA toolkit part-entry vector position 8a
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100618YCe1717 HC_Kan_RFP-p8bEMMA toolkit part-entry vector position 8b
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100619YCe1699 HC_Kan_RFP-p9EMMA toolkit part-entry vector position 9
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100620YCe1701 HC_Kan_RFP-p10EMMA toolkit part-entry vector position 10
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100621YCe1703 HC_Kan_RFP-p11EMMA toolkit part-entry vector position 11
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100622YCe1705 HC_Kan_RFP-p12EMMA toolkit part-entry vector position 12
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100623YCe1707 HC_Kan_RFP-p13EMMA toolkit part-entry vector position 13
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100624YCe1709 HC_Kan_RFP-p14EMMA toolkit part-entry vector position 14
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100625YCe1719 HC_Kan_RFP-p15EMMA toolkit part-entry vector position 15
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100626YCe1486 HC_Kan_RFP-p16EMMA toolkit part-entry vector position 16
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100627YCe1487 HC_Kan_RFP-p17EMMA toolkit part-entry vector position 17
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100628YCe1488 HC_Kan_RFP-p18EMMA toolkit part-entry vector position 18
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100629YCe1491 HC_Kan_RFP-p19EMMA toolkit part-entry vector position 19
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100630YCe1711 HC_Kan_RFP-p20EMMA toolkit part-entry vector position 20
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100631YCe1713 HC_Kan_RFP-p21EMMA toolkit part-entry vector position 21
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100632YCe1514 HC_Kan_RFP-p22EMMA toolkit part-entry vector position 22
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100633YCe1715 HC_Kan_RFP-p23EMMA toolkit part-entry vector position 23
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100634YCe1731 HC_Kan_RFP-p24EMMA toolkit part-entry vector position 24
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100635YCe1721 HC_Kan_RFP-p25EMMA toolkit part-entry vector position 25
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100636YCe2764 HC_Kan_RFP-Connector entry vectorEMMA toolkit connector-entry vector
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100637YCe3736_HC_Amp_ccdB_receiver vectorEMMA toolkit receiver vector
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100638YCe3731 HC_Kan_conn_A-BEMMA toolkit Assembly connector A-B
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100639YCe3732 HC_Kan_conn_B-CEMMA toolkit Assembly connector B-C
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100640YCe1915 HC_Kan_conn_A-CEMMA toolkit Assembly connector A-C
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100641YCe2024 HC_Kan_conn_D-EEMMA toolkit Assembly connector D-E
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100642YCe3756 HC_Kan_conn_E-FEMMA toolkit Assembly connector E-F
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100643YCe1917 HC_Kan_conn_D-FEMMA toolkit Assembly connector D-F
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100644YCe2982 HC_Kan_conn_B-E EMMA toolkit Assembly connector B-E
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100645YCe2984 HC_Kan_conn_B-LEMMA toolkit Assembly connector B-L
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100646YCe1921 HC_Kan_conn_H-KEMMA toolkit Assembly connector H-K
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100647YCe2986 HC_Kan_conn_H-JEMMA toolkit Assembly connector H-J
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100648YCe3734 HC_Kan_conn_Ha-JEMMA toolkit Assembly connector Ha-J
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100649YCe3735 HC_Kan_conn_Ha-KEMMA toolkit Assembly connector Ha-K
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100650YCe1913 HC_Kan_conn_H-HaEMMA toolkit Assembly connector H-Ha
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100651YCe2022 HC_Kan_conn_J-KEMMA toolkit Assembly connector J-K
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100652YCe3760 HC_Kan_conn_L-MEMMA toolkit Assembly connector L-M
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100653YCe2718 HC_Kan_conn_L-NEMMA toolkit Assembly connector L-N
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100654YCe1920 HC_Kan_conn_L-REMMA toolkit Assembly connector L-R
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100655YCe2731 HC_Kan_conn_L-WEMMA toolkit Assembly connector L-W
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100656YCe1918 HC_Kan_conn_L-YEMMA toolkit Assembly connector L-Y
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100657YCe3733 HC_Kan_conn_M-REMMA toolkit Assembly connector M-R
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100658YCe3761 HC_Kan_conn_N-REMMA toolkit Assembly connector N-R
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100659YCe2020 HC_Kan_conn_Q-REMMA toolkit Assembly connector Q-R
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100660YCe3762 HC_Kan_conn_Q-WEMMA toolkit Assembly connector Q-W
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100661YCe2780 HC_Kan_conn_R-WEMMA toolkit Assembly connector R-W
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100662YCe2786 HC_Kan_conn_T-VEMMA toolkit Assembly connector T-V
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100663YCe3757 HC_Kan_conn_U-VEMMA toolkit Assembly connector U-V
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100664YCe2721 HC_Kan_conn_W-ZEMMA toolkit Assembly connector W-Z
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100665YCe2733 HC_Kan_conn_W-YEMMA toolkit Assembly connector W-Y
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100666YCe3159 HC_Kan_conn_W-XEMMA toolkit Assembly connector W-X
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100667YCe2066 HC_Kan_conn_Y-ZEMMA toolkit Assembly connector Y-Z
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100668YCe4520 HC_Kan_conn_BsaI-1_p1EMMA toolkit Assembly connector for second step assembly
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100669YCe2725 HC_Kan_conn_BsaI-2_p24EMMA toolkit Assembly connector for second step assembly
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100670YCe4522 HC_Kan_conn_BsaI-3_p1EMMA toolkit Assembly connector for second step assembly
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100671YCe2730 HC_Kan_conn_BsaI-4_p25EMMA toolkit Assembly connector for second step assembly
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100672YCe1759 HC_Kan_CAGp_p3part designed to occupy position 3 of EMMA. Functional category: Constitutional promoter
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100673YCe1760 HC_Kan_IRES2_p8bpart designed to occupy position 8a of EMMA. Functional category: IRES sequence
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100674YCe1762 HC_Kan_SV40p_p14part designed to occupy position 14 of EMMA. Functional category: Constitutional promoter
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100675YCe1883 HC_Kan_p2A_p8part designed to occupy position 8a of EMMA. Functional category: p2A sequence
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100676YCe1885 HC_Kan_Kozak-ATG-MLS_p6part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting
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100678YCe1889 HC_Kan_SV40-polyA_p11part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence
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100679YCe1891 HC_Kan_SV40-ORI_p25part designed to occupy position 25 of EMMA. Functional category: Viral origin of replication
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100680YCe1895 HC_Kan_Kozak-ATG_p6part designed to occupy position 6 of EMMA. Functional category: Kozak sequence
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100681YCe1897 HC_Kan_C-KDEL_8apart designed to occupy position 8a of EMMA. Functional category: Signal peptide for subcellular targeting
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100682YCe1899 HC_Kan_PuroR_p9part designed to occupy position 9 of EMMA. Functional category: Selection marker
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100683YCe1901 HC_Kan_PGKpA_p22part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence
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100684YCe1903 HC_Kan_SV40-NLS_p6part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting
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100685YCe1905 HC_Kan_DmrA_p21part designed to occupy position 21 of EMMA. Functional category: Dimerization domain
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100686YCe1911 HC_Kan_Insulator_FB_p2part designed to occupy position 22 of EMMA. Functional category: Insulator
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100687YCe2026 HC_Kan_mRuby2_p9part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker
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100688YCe2030 HC_Kan_bGHpA_p16part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence
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100689YCe2034 HC_Kan_mTagBFP2_p9part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker
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100690YCe2038 HC_Kan_CMVp_p18part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter
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100691YCe2040 HC_Kan_PuroR_p21part designed to occupy position 21 of EMMA. Functional category: Selection marker
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100692YCe2046 HC_Kan_PuroR_p15part designed to occupy position 15 of EMMA. Functional category: Selection marker
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100693YCe2052 HC_Kan_DmrC_p9part designed to occupy position 9 of EMMA. Functional category: Dimerization domain
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100694YCe2058 HC_Kan_Kozak_ATG_Palm_p6part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting
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100695YCe2060 HC_Kan_Linker3_p20part designed to occupy position 20 of EMMA. Functional category: Peptide linker
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100696YCe2064 HC_Kan_p2A_p20part designed to occupy position 20 of EMMA. Functional category: p2A sequence
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100697YCe2735 HC_Kan_Kozak-ATG-IgkL_p6part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting
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100698YCe2741 HC_Kan_EF1ap_p18part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter
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100699YCe2746 HC_Kan_Linker1_p8part designed to occupy position 8 of EMMA. Functional category: Peptide linker
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100700YCe2748 HC_Kan_5'ITR-PB_p1part designed to occupy position 1 of EMMA. Functional category: Inverted Terminal Repeat
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100701YCe2750 HC_Kan_3'ITR-PB_p24part designed to occupy position 24 of EMMA. Functional category: Inverted Terminal Repeat
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100702YCe3752 HC_Kan_IRES2_p20part designed to occupy position 20 of EMMA. Functional category: IRES sequence
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100703YCe3766 HC_Kan_3'-LTR_p10part designed to occupy position 10 of EMMA. Functional category: Long Terminal Repeat
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100704YCe3777 HC_Kan_Chimeric-5'LTR_p1part designed to occupy position 1 of EMMA. Functional category: Long Terminal Repeat
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Recombinant Antibodies from Article