YCe2741 HC_Kan_EF1ap_p18
(Plasmid #100698)

• Purpose: part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter
• Depositing Lab: Yizhi Cai
• Publication: Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.

Backbone

• Vector backbone: pSMART HC_Kan
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1a promoter
• Species: H. sapiens (human)
• Entrez Gene: EEF1A1 (a.k.a. CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • YCe2741 HC_Kan_EF1ap_p18.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

strong constitutive promoter derived from endogenous promoter of human elongation factor EF-1? . EF-1 alpha is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells)