PurposeEncodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123808||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MTK3b_030 was a gift from Hana El-Samad (Addgene plasmid # 123808 ; http://n2t.net/addgene:123808 ; RRID:Addgene_123808)
For your References section:A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. 10.1021/acssynbio.9b00322 PubMed 31686495