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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
(Plasmid #158962)


Item Catalog # Description Quantity Price (USD)
Plasmid 158962 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hsp70l promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)

Gene/Insert 2

  • Gene/Insert name
    floxed DsRed cassette
  • Species
  • Insert Size (bp)

Gene/Insert 3

  • Gene/Insert name
  • Species
  • Insert Size (bp)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP was a gift from Michael Brand (Addgene plasmid # 158962 ; ; RRID:Addgene_158962)
  • For your References section:

    Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish. Hans S, Zoller D, Hammer J, Stucke J, Spiess S, Kesavan G, Kroehne V, Eguiguren JS, Ezhkova D, Petzold A, Dahl A, Brand M. Nat Commun. 2021 Feb 18;12(1):1125. doi: 10.1038/s41467-021-21427-6. 10.1038/s41467-021-21427-6 PubMed 33602923