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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA
(Plasmid #108871)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108871 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Tol2
  • Backbone size w/o insert (bp) 5853
  • Total vector size (bp) 13822
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Due to size of vector, bacteria are slow growing. Grow larger cultures for high plasmid recovery.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    5xU6:sgRNA
  • Species
    D. rerio (zebrafish), Synthetic
  • Insert Size (bp)
    2693
  • Promoter U6

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer ACATTAAATGAAATGCATcagt
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cas9-t2A-GFP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5276
  • Promoter hsp70

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA was a gift from Alex Schier (Addgene plasmid # 108871 ; http://n2t.net/addgene:108871 ; RRID:Addgene_108871)
  • For your References section:

    Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain. Raj B, Wagner DE, McKenna A, Pandey S, Klein AM, Shendure J, Gagnon JA, Schier AF. Nat Biotechnol. 2018 Mar 28. pii: nbt.4103. doi: 10.1038/nbt.4103. 10.1038/nbt.4103 PubMed 29608178