164,388 results

1. pDONR221-SLC7A5_STOP

   Plasmid

   #161363

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A5 (SLC7A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAL119-TK

   Plasmid

   #21911

   Depositor

   Maria Castro

   Article

   Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

   Insert

   HSV Type 1 thymidine kinase
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. OmpA-mNG-sfTq2ox

   Plasmid

   #124220

   Purpose

   Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   OmpA-mNG-sfTq2ox
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LRRC3_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176972

   Purpose

   Mammalian expression vector encoding LRRC3 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   LRRC3 (LRRC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTMVi-N

   Plasmid

   #118756

   Purpose

   Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus cDNA fragment with a cloning polylinker
   

   Use

   Intermediate to build tobacco mosaic virus recomb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-N-Flag-NLRP3 91-710

   Plasmid

   #75140

   Purpose

   Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 91-710

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIRT5 Flag

   Plasmid

   #13816

   Purpose

   Mammalian expression of human SIRT5 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT5 (SIRT5 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-HA-deLACCO1-NGR

    Plasmid

    #208024

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPEgRNA

    Plasmid

    #172716

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO human shRNA 2 DEPTOR

    Plasmid

    #21336

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 2 (DEPTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

    Plasmid

    #30505

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucuronidase (gusA Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPOP-V5 (F133V)

    Plasmid

    #169725

    Purpose

    Mammalian Expression of mouse SPOP F133V

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    F133V

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHnCBS1D

    Plasmid

    #52065

    Purpose

    expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

    Depositor

    David Savage

    Article

    Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

    Insert

    CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEH343 CD63 MS2 HA

    Plasmid

    #168217

    Purpose

    Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX-Baf155

    Plasmid

    #25856

    Depositor

    Hans Schöler

    Article

    Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

    Insert

    Brg1 associated factor 155 (Smarcc1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only