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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_NFIC

Plasmid

#187739

Purpose

MAC-tagged gene expression of human NFIC

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

NFIC (NFIC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPD189 VP16-ZF37

Plasmid

#138759

Purpose

Constitutive expression of VP16-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBAM1

Plasmid

#60487

Purpose

This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-URA3

Plasmid

#130875

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2

Depositor

Chang Liu

Article

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 wt

Plasmid

#120313

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-rtTA

Plasmid

#175274

Purpose

AAV vector mediating neuron-specific expression of rtTA

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Reverse tetracycline-controlled transactivator (rtTA)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.eGFP.WPRE.hGH

Plasmid

#105549

Purpose

AAV expression of EGFP from GFAP promoter

Depositor

James M. Wilson

Has Service

AAV5

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPD559 ZF173x6-Compact_EYFP

Plasmid

#138873

Purpose

EYFP reporter vector for the ZF173x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF173x6-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF173x6-Compact

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPD539 VP16-ZF173

Plasmid

#138773

Purpose

Constitutive expression of VP16-ZF173 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF173

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD526 VP16-ZF13

Plasmid

#138755

Purpose

Constitutive expression of VP16-ZF13 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF13

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD547 ZF43x6-Compact_EYFP

Plasmid

#138861

Purpose

EYFP reporter vector for the ZF43x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x6-Compact

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PCTK3

Plasmid

#20562

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCTK3 (CDK18 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET28b-zDjCas13d-His

Plasmid

#234481

Purpose

Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DjCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Tags

Expression

Mutation

Promoter

PGK

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNC02

Plasmid

#233644

Purpose

For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

sfGFP with c-terminal VitelloTagB

Use

Tags

6-His

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#233642

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagB

Use

Tags

6-His

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT57

Plasmid

#223429

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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