FUW-TetON-Sox10
(Plasmid #115242)

• Purpose: Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)
• Depositing Lab: Catherine Verfaillie
• Publication: Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.

Backbone

• Vector backbone: FUW TetON (AddGene plasmid etO-FUW-OSKM Plasmid #20321)
• Backbone manufacturer: Whitehead Institute
• Backbone size w/o insert (bp): 8400
• Total vector size (bp): 9783
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOX10
• Alt name: SRY-box 10
• Species: H. sapiens (human)
• Insert Size (bp): 1401
• GenBank ID: NM_006941.3
• Entrez Gene: SOX10 (a.k.a. DOM, PCWH, SOX-10, WS2E, WS4, WS4C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry