PurposeMammalian expression 8xHis-Ub
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107392||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert name8 tandem repeats of 6xHis-Ub (See comments below)
SpeciesH. sapiens (human)
Insert Size (bp)2200
- Promoter CMV
/ Fusion Protein
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid contains 8 6xHis Ub repeats that could not be completely sequenced with Next Generation Sequencing. Restriction digest with NheI and KpnI confirms the 8 repeats with a 2.2 kb band.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-8xHis-Ub was a gift from William Kaelin (Addgene plasmid # 107392 ; http://n2t.net/addgene:107392 ; RRID:Addgene_107392)
For your References section:The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK, Bradner JE, Kaelin WG Jr. Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. Epub 2013 Nov 29. 10.1126/science.1244917 PubMed 24292623