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A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.
Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES
Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 10.1038/s41589-018-0004-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108409pmiRFP-N1Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid
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108410pBAD-HisD-miRFPMonomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid
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108411pmiRFP-H2B-C1Histone 2B fused to monomeric near-infrared fluorescent protein miRFP
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108412pmiRFP-beta-Actin-C-19Beta-actin fused to monomeric near-infrared fluorescent protein miRFP
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108413pmiRFP-alpha-Tubulin-19-CAlpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP
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108414pmiRFP-Vimentin-17-CVimentin fused to monomeric near-infrared fluorescent protein miRFP
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108415pTol2-UAS-zmiRFPCodon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid
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108416pAAV-Syn-miRFPMonomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid
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108417pAAV-CaMKII-Archon1-EGFPAAV production plasmid encoding for Archon1 fluorescent voltage reporter
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108418pAAV-CaMKII-Archon1-KGC-EGFPAAV production plasmid encoding for Archon1 fluorescent voltage reporter
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108419pAAV-CaMKII-Archon1-KGC-EGFP-ER2AAV production plasmid encoding for Archon1 fluorescent voltage reporter
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108420pAAV-CAG-Archon1-KGC-EGFP-ER2AAV production plasmid encoding for Archon1 fluorescent voltage reporter
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108421pAAV-CAG-Archon2-KGC-EGFP-ER2AAV production plasmid encoding for Archon2 fluorescent voltage reporter
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108422pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression
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108423pCAG-Archon1-KGC-EGFP-ER2-WPREArchon1 fluorescent voltage reporter in mammalian expression plasmid
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108424pCAG-Archon2-KGC-GFP-ER2-WPREArchon2 fluorescent voltage reporter in mammalian expression plasmid
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108425pHR-EF1a-Archon1-KGC-EGFP-ER2Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter
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108426pHR-EF1a-Archon2-KGC-EGFP-ER2Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter
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108427pTol2-UAS-zArchon1-KGC-EGFP-ER2Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid
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108428pTol2-UAS-zArchon2-KGC-EGFP-ER2Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid
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108429pSF143-wArchon1-KGC-EGFP-ER2Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid
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108430pSF143-wArchon2-KGC-EGFP-ER2Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid
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115890pAAV-EF1α1.1-Archon1-KGC-GFP-ER2AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.
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115891pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
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115892pAAV-Syn-Archon1-KGC-GFP-ER2AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.
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115893pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
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