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A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination.
Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD
Cell. 2017 Mar 23;169(1):108-119.e20. doi: 10.1016/j.cell.2017.03.006.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
89344DNA-CARzeta-GFP2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP
89345DNA-CARzeta-IRESpuro2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette
89346DNA-CARtcr-IRESpuro2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
89347TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

Antibodies from Article